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首页> 外文期刊>Environmental Science & Technology >Prion degradation in soil: Possible role of microbial enzymes stimulated by the decomposition of buried carcasses
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Prion degradation in soil: Possible role of microbial enzymes stimulated by the decomposition of buried carcasses

机译：病毒在土壤中的降解：埋藏尸体分解刺激微生物酶的可能作用

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摘要

This study is part of a European project focused on understanding the biotic and abiotic mechanisms involved in the retention and dissemination of transmissible spongiform encephalopathies (TSE) infectivity in soil in order to propose practical recommendations to limit environmental contamination. A 1-year field experiment was conducted with lamb carcasses buried in a pasture soil at three depths (25, 45, and 105 cm). Microbial community response to carcasses was monitored through the potential proteolytic activity and substrate induced respiration (SIR). Soil above carcasses and control soil exhibited low proteolytic capacity, whatever the depth of burial. Contrastingly, in soil beneath the carcasses, proteolysis was stimulated. Decomposing carcasses also stimulated SIR, i.e., microbial biomass, suggesting that proteolytic populations specifically developed on lixiviates from animal tissues. Decomposition of soft tissues occurred within 2 months at subsurface while it lasted at least 1 year at deeper depth where proteolytic activities were season-dependent. The ability of soil proteases to degrade the beta form of prion protein was shown in vitro and conditions of burial relevant to minimize the risk of prion protein dissemination are discussed.
机译：这项研究是一项欧洲项目的一部分，该项目的重点是了解与土壤中可传播的海绵状脑病（TSE）感染力的保持和传播有关的生物和非生物机制，以便提出切实可行的建议来限制环境污染。进行了为期一年的野外试验，将羔羊尸体埋在三个深度（25、45和105厘米）的牧场土壤中。通过潜在的蛋白水解活性和底物诱导的呼吸（SIR）来监测微生物对community体的反应。无论埋葬深度如何，car体上方的土壤和对照土壤的蛋白水解能力均较低。相反，在car体下方的土壤中，蛋白水解受到刺激。尸体的分解也刺激了SIR，即微生物生物量，这表明从动物组织中浸出的蛋白水解种群特别发达。软组织的分解在地下2个月内发生，而在更深的深度至少持续1年，而蛋白水解的活动取决于季节。在体外显示了土壤蛋白酶降解the病毒蛋白β形式的能力，并讨论了将to病毒蛋白传播的风险降至最低有关的埋葬条件。

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