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首页> 外文期刊>Environmental Science & Technology >Determination of microbial carbon sources in petroleum contaminated sediments using molecular C-14 analysis
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Determination of microbial carbon sources in petroleum contaminated sediments using molecular C-14 analysis

机译：使用分子C-14分析测定石油污染沉积物中的微生物碳源

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Understanding microbial carbon sources is fundamental to elucidating the role of microbial communities in carbon cycling and in the biodegradation of organic contaminants. Because the majority of anthropogenic contaminants are either directly or indirectly derived from fossil fuels that are devoid of C-14, radiocarbon can be used as a natural inverse tracer of contaminant carbon in the contemporary environment. Here, C-14 analysis of individual microbial phospholipid fatty acids (PLFA) was used to characterize the carbon sources utilized by the active microbial community in salt marsh sediments contaminated by the Florida oil spill of 1969 in Wild Harbor, West Falmouth, MA. A specific goal was to determine whether this community is actively degrading petroleum residues that persist in these sediments. The Delta(14)C values of microbial PLFA in all sediment horizons (contaminated and noncontaminated) matched the Delta(14)C of the total sedimentary organic carbon after petroleum removal, indicating that no measurable metabolism of petroleum residues was occurring. This result agrees with ancillary data such as the delta(13)C content and distribution of PLFA, and the residual hydrocarbon composition determined by comprehensive two-dimensional gas chromatography (GCxGC) analysis. We hypothesize that microbes have chosen to respire the natural organic matter rather than the residual petroleum hydrocarbons because the former is more labile. Future efforts directed at determining indices of microbial degradation of petroleum hydrocarbons should consider competition with natural organic matter.
机译：了解微生物碳源是阐明微生物群落在碳循环和有机污染物生物降解中的作用的基础。由于大多数人为污染物是直接或间接地来自不含C-14的化石燃料，因此放射性碳可以用作当代环境中污染物碳的自然逆向示踪剂。在这里，对单个微生物磷脂脂肪酸（PLFA）的C-14分析被用来表征活性微生物群落利用盐沼沉积物中的碳源，该盐沼沉积物受到1969年佛罗里达州马萨诸塞州西法尔茅斯的怀尔德海港漏油事件的污染。一个具体目标是确定该社区是否正在积极降解残留在这些沉积物中的石油残留物。在去除沉积物后，所有沉积层（受污染和未污染）中微生物PLFA的Delta（14）C值与总沉积有机碳的Delta（14）C匹配，表明没有发生可测量的石油残留代谢。该结果与辅助数据如PLFA的delta（13）C含量和分布以及通过全面二维气相色谱（GCxGC）分析确定的残余烃组成相符。我们假设微生物选择呼吸自然的有机物质，而不是残留的石油碳氢化合物，因为前者更不稳定。针对确定石油碳氢化合物微生物降解指标的未来工作，应考虑与天然有机物的竞争。

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