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首页> 外文期刊>Environmental Science & Technology >Settling behavior of unpurified Cryptosporidium oocysts in laboratory settling columns
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Settling behavior of unpurified Cryptosporidium oocysts in laboratory settling columns

机译:未纯化的隐孢子虫卵囊在实验室沉降柱中的沉降行为

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The settling behavior of fresh and aged unpurified oocysts was examined in settling column suspensions with varied ionic strengths and concentrations of calcium and magnesium. Independent measurements of the size and density of unpurified oocysts were performed to determine a theoretical settling velocity for the test populations. Viability of the oocysts was assessed using a dye permeability assay, Latex microspheres were included to provide a standard by which to assess the settling conditions in the columns. Mean settling velocities for viable oocysts measured in this work were faster than predicted and faster than measured for purified oocysts in other work: 1.31 (+/- 0.21) mu m/s for viable oocysts from populations having a low percentage of viable oocysts and 1.05 (+/- 0.20) mu m/s for viable oocysts from populations with a high percentage of viable oocysts. Results were attributed to the higher than previously reported densities measured for oocysts in this study and the presence of fecal material, which allowed opportunity for particle agglomeration. Settling velocity of oocysts was significantly related to the viability of the population, particle concentration, ionic strength, and presence of calcium and magnesium in the suspending medium. Behavior of the latex microspheres was not entirely predictive of the behavior of the oocysts under the test conditions. Viable oocysts may have a greater probability of settling than previously assumed; however, nonviable, and especially nonintact, oocysts have the potential to be significantly transported in water. This work underscores the importance of assessing the viability of oocysts to predict their response to environmental and experimental conditions.
机译:在具有不同离子强度以及钙和镁浓度的沉降柱悬浮液中,检查了新鲜的和老化的未纯化卵囊的沉降行为。对未纯化卵囊的大小和密度进行了独立测量,以确定测试群体的理论沉降速度。使用染料渗透性测定法评估卵囊的生存力,其中包括Latex微球,以提供一种标准,用以评估色谱柱中的沉降条件。在这项工作中测得的活卵囊的平均沉降速度快于预期,并且在其他工作中比纯化后的卵囊测得的速度快:活卵囊百分比低的人群的活卵囊的沉降速度为1.31(+/- 0.21)m / s,1.05为1.05卵囊百分比高的人群中的卵囊的(+/- 0.20)μm / s。结果归因于这项研究中测得的卵囊密度高于先前报道的密度,并且粪便物质的存在为颗粒聚团提供了机会。卵囊的沉降速度与种群的生存力,颗粒浓度,离子强度以及悬浮介质中钙和镁的存在密切相关。乳胶微球的行为不能完全预测测试条件下卵囊的行为。可行的卵囊可能比以前认为的有更大的沉降可能性。然而,不可行的卵囊,尤其是非完整的卵囊,有可能在水中大量运输。这项工作强调了评估卵囊生存能力以预测其对环境和实验条件的反应的重要性。

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