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首页> 外文期刊>Environmental Science & Technology >Development of an immunoassay for the determination of polyaromatic hydrocarbons in plasma samples from oiled seabirds
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Development of an immunoassay for the determination of polyaromatic hydrocarbons in plasma samples from oiled seabirds

机译：免疫测定法测定油样海鸟血浆样品中多环芳烃的进展

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External exposure assessment of oiled seabirds is undertaken by assessment of the percentage oil coverage of the plumage. Nondestructive monitoring of the toxic fraction of petroleum oils and diesels (polyaromatic hydrocarbons, PAHs) which enters the general circulation (internal exposure burden) of oiled seabirds is rarely undertaken. This is because the traditionally used chromatographic methods for plasma PAH analysis require larger sample volumes than those that can be safely collected from smaller species, such as guillemots (Uria aalge). Furthermore, these methods are not a cost-effective or practical approach for analysis of large numbers of birds in a short time period as part of an oil spill response in wildlife rehabilitation centers. This study describes the modification and validation of a commercially available PAH immunoassay (cRaPID PAH) to enable high-throughput, cost-effective, simple, and rapid determination of total PAH concentrations in 50 mu L volumes of plasma. The limit of detection of the assay was 0.1 ng/mL as benzo-alpha-pyrene (BaP) equivalents with a working range of 0.120 ng/mL. As further validation of the immunoassay, PAHs were determined by GC-MS. GC-MS data were significantly positively correlated with corresponding immunoassay data for the same birds (r(2) = 0.976, p < 0.001). The plasma PAH concentrations of 40 oiled guillemots; stranded on U.K. shores were determined using the assay to demonstrate its usefulness for biomonitoring studies. The mean Sigma PAH concentration observed was 1.05 +/- 0.67 ppm (range 0.02-2.40 pprn as BaP equivalents). The modifications to the cRaPID PAH kit in this study enable nondestructive, high-throughput, semiquantitative determination of PAH concentrations in plasma samples suitable for exposure assessment of oiled seabirds during oil spill response and rehabilitation.
机译：涂油海鸟的外部暴露评估是通过评估羽毛的油覆盖百分比来进行的。很少对进入加油海鸟的一般循环（内部接触负担）的石油和柴油（聚芳烃，PAHs）的有毒成分进行无损监测。这是因为传统上用于血浆PAH分析的色谱方法需要比可以从较小的物种（例如海雀科的小鸟（Uria aalge））安全地收集的更大的样品量。此外，作为野生动植物康复中心漏油事件的一部分，这些方法不是在短时间内分析大量鸟类的经济有效方法。这项研究描述了对市售PAH免疫测定法（cRaPID PAH）的修改和验证，以实现高通量，经济高效，简单而快速地测定50μL血浆中总PAH浓度。该测定的检出限为0.1 ng / mL，当量为0.120 ng / mL的苯并-α-py（BaP）当量。为了进一步验证免疫分析结果，通过GC-MS测定了PAH。 GC-MS数据与相同禽类的相应免疫测定数据显着正相关（r（2）= 0.976，p <0.001）。血浆油中PAH的浓度为40只油海胆；使用测定法测定了在英国海岸上搁浅的链，以证明其对生物监测研究的有用性。观察到的平均Sigma PAH浓度为1.05 +/- 0.67 ppm（BaP当量范围为0.02-2.40 pprn）。通过对本研究中的cRaPID PAH试剂盒进行的改进，可以无损，高通量，半定量测定血浆样品中的PAH浓度，适用于在溢油应急响应和恢复过程中对涂油海鸟进行暴露评估。

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