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首页> 外文期刊>Environmental Science & Technology >Miniaturized lead sensor based on lead-specific DNAzyme in a manocapillary interconnected microfluidic device
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Miniaturized lead sensor based on lead-specific DNAzyme in a manocapillary interconnected microfluidic device

机译：基于毛细血管互连微流体装置中铅特异性DNA酶的微型铅传感器

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A miniaturized lead sensor has been developed by combining a lead-specific DNAzyme with a microfabricated device containing a network of microfluidic channels that are fluidically coupled via a nanocapillary array interconnect. A DNAzyme construct, selective for cleavage in the presence of Pb2+ and derivatized with fluorophore (quencher) at the 5' (3') end of the substrate and enzyme strands, respectively, forms a molecular beacon that is used as the recognition element. The nanocapillary array membrane interconnect is used to manipulate fluid flows and deliver the small-volume sample to the beacon in a spatially confined detection window where the DNAzyme is interrogated using laser-induced fluorescence detection. A transformed log plot of the fluorescent signal exhibits a linear response (r(2) = 0.982) over a Pb2+ concentration range of 0.1-100 mu M, and a detection limit of 11 nM. The sensor has been applied to the determination of Pb2+ in an electroplating sludge reference material, the result agreeing with the certified value within 4.9 %. Quantitative measurement of Pb2+ in this complex sample demonstrates the selectivity of this sensor scheme and points favorably to the application of such technologies to analysis of environmental samples. The unique combination of a DNAzyme with a microfluidic-nanofluiclic hybrid device makes it possible to change the DNAzyme to select for other compounds of interest, and to incorporate multiple sensing systems within a single device for greater flexibility. This work represents the initial steps toward creation of a robust field sensor for lead in groundwater or drinking water.
机译：通过将铅特异性DNA酶与微制造的设备相结合，开发了一种小型的铅传感器，该微制造的设备包含通过纳米毛细管阵列互连流体耦合的微流体通道网络。在Pb2 +存在下选择性切割并在底物和酶链的5'（3'）末端分别用荧光团（淬灭剂）衍生的DNAzyme构建体形成了分子信标，用作识别元件。纳米毛细管阵列膜互连件用于操纵流体流动，并将小体积样品输送到空间受限的检测窗口中的信标中，在该窗口中，使用激光诱导的荧光检测来询问DNA酶。荧光信号的变换对数图在0.1-100μM的Pb2 +浓度范围内显示线性响应（r（2）= 0.982），检测极限为11 nM。该传感器已用于电镀污泥标准物质中Pb2 +的测定，结果与4.9％内的认证值相符。对该复杂样品中Pb2 +的定量测量证明了该传感器方案的选择性，并有利地指出了此类技术在环境样品分析中的应用。 DNAzyme与微流体-纳米流体混合设备的独特结合使得可以改变DNAzyme来选择其他感兴趣的化合物，并将多个传感系统整合到单个设备中以提高灵活性。这项工作代表了创建用于地下水或饮用水中铅的坚固型现场传感器的初步步骤。

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