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首页> 外文期刊>Environmental Science & Technology >Link between chemotactic response to Ni2+ and its adsorption onto the Escherichia coli cell surface
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Link between chemotactic response to Ni2+ and its adsorption onto the Escherichia coli cell surface

机译：对Ni2 +的趋化反应与其吸附到大肠杆菌细胞表面之间的联系

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摘要

Bacterial chemotaxis is of medical, biological, and geological significance. Despite its importance, current chemotaxis measurements fail to account for the speciation of the chemical effector and the protonation state of the bacterial surface. We hypothesize that adsorption of Ni2+ onto the surface of Escherichia coli can influence its effective concentration and therefore influence its ability to induce a repellent response. By measuring repellent response at different pH values, the influence of Ni2+ adsorption on chemotaxis was assessed. In addition, we tested the effect of different Ni2+ chelating agents. Our data indicate that adsorption reactions influence the chemotactic response to Ni2+. We use potentiometric titration and Ni2+ adsorption experiments to develop and constrain a thermodynamic model capable of quantifying the concentration of Ni2+ at the bacteria/solution interface. Results from this model predict that the concentration of adsorbed Ni2+ is linearly proportional to the magnitude of the chemotactic response in E coli. If adsorption is linked to chemotaxis in other cases, then chemotactic responses in realistic settings depend on a number of environmental factors such as pH, competing binding agents (e.g., aqueous organic acids, natural organic matter, mineral surfaces, etc.), and ionic strength, Our modeling approach quantifies adsorbed species on bacterial surfaces and may be used to predict the responses of different species to a variety of chemoeffectors. Our data suggest that specified changes in environmental conditions can be used to tune chemotactic responses in natural biological and geological settings.
机译：细菌趋化性具有医学，生物学和地质意义。尽管其重要性，当前的趋化性测量不能解决化学效应子的种类和细菌表面的质子化状态。我们假设Ni2 +在大肠杆菌表面的吸附会影响其有效浓度，因此会影响其诱导排斥反应的能力。通过测量在不同pH值下的驱避剂反应，评估了Ni2 +吸附对趋化性的影响。此外，我们测试了不同的Ni2 +螯合剂的作用。我们的数据表明吸附反应影响对Ni2 +的趋化反应。我们使用电位滴定法和Ni2 +吸附实验来开发和约束能够量化细菌/溶液界面处Ni2 +浓度的热力学模型。该模型的结果表明，吸附的Ni2 +浓度与大肠杆菌中趋化反应的强度成线性比例。如果在其他情况下吸附与趋化性相关，那么现实环境中的趋化性响应取决于许多环境因素，例如pH，竞争性结合剂（例如，水性有机酸，天然有机物，矿物表面等）和离子性强度，我们的建模方法可以量化细菌表面吸附的物种，可用于预测不同物种对各种化学效应物的响应。我们的数据表明，特定的环境条件变化可用于调整自然生物学和地质环境中的趋化反应。

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