Bacterial bioassay for rapid and accurate analysis of arsenic in highly variable groundwater samples

Trang PTK; Berg M; Viet PH; Van Mui N; Van Der Meer JR

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Bacterial bioassay for rapid and accurate analysis of arsenic in highly variable groundwater samples

机译：细菌生物测定法可快速准确地分析高度变化的地下水样品中的砷

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In this study, we report the first ever large-scale environmental validation of a microbial reporter-based test to measure arsenic concentrations in natural water resources. A bioluminescence-producing arsenic-inducible bacterium based on Escherichia coli was used as the reporter organism. Specific protocols were developed with the goal to avoid the negative influence of iron in groundwater on arsenic availability to the bioreporter cells. A total of 194 groundwater samples were collected in the Red River and Mekong River Delta regions of Vietnam and were analyzed both by atomic absorption spectroscopy (AAS) and by the arsenic bioreporter protocol. The bacterial cells performed well at and above arsenic concentrations in groundwater of 7 mu g/L, with an almost linearly proportional increase of the bioluminescence signal between 10 and 100 mu g As/L (r(2) = 0.997). Comparisons between AAS and arsenic bioreporter determinations gave an overall average of 8.0% false negative and 2.4% false positive identifications for the bioreporter prediction at the WHO recommended acceptable arsenic concentration of 10 mu g/L, which is far better than the performance of chemical field test kits. Because of the ease of the measurement protocol and the low application cost, the microbiological arsenic test has a great potential in large screening campaigns in Asia and in other areas suffering from arsenic pollution in groundwater resources.

机译：在这项研究中，我们报告了首次以微生物报告为基础的大规模环境验证，该测试可测量天然水资源中的砷浓度。基于大肠杆菌的产生生物发光的砷诱导细菌被用作报告生物。为了避免地下水中铁对生物报告细胞中砷的可利用性的不利影响，制定了特定的方案。在越南的红河和湄公河三角洲地区共收集了194个地下水样品，并通过原子吸收光谱法（AAS）和砷生物报告程序进行了分析。细菌细胞在砷浓度等于或高于7μg/ L的地下水中表现良好，并且生物发光信号在10和100μgAs / L之间几乎呈线性比例增加（r（2）= 0.997）。在世界卫生组织建议的可接受的砷浓度为10μg / L的情况下，对AAS和砷生物报告者测定值的比较得出的生物报告者预测的总体平均值为8.0％假阴性和2.4％假阳性，远优于化学领域的性能测试套件。由于测量协议的简便性和低廉的应用成本，微生物砷测试在亚洲及其他受到地下水砷污染的其他地区的大型筛查活动中具有巨大的潜力。

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来源
《Environmental Science & Technology》 |2005年第19期|p. 7625-7630|共6页
作者
Trang PTK; Berg M; Viet PH; Van Mui N; Van Der Meer JR;
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作者单位

Hanoi Univ Sci, Fac Biol, Hanoi, Vietnam;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类环境化学;
关键词
SENSING SYSTEMS; DRINKING-WATER; CONTAMINATION; PRESERVATION; BANGLADESH; VIETNAM; MERCURY; ACID;

机译：传感系统;饮用水;污染;保留;孟加拉国;越南;汞;酸;

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1. Highly accurate radiochemical neutron activation analysis of arsenic in biological materials involving selective isolation of arsenic by hybrid and conventional ion exchange [J] . Ewelina Chajduk, Rajmund S. Dybczyński Microchimica Acta . 2010,第1a2期

机译：生物材料中砷的高精度放射化学中子活化分析，涉及通过混合和常规离子交换选择性分离砷
2. Highly accurate radiochemical neutron activation analysis of arsenic in biological materials involving selective isolation of arsenic by hybrid and conventional ion exchange [J] . Ewelina Chajduk, Rajmund S. Dybczynski Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis . 2010,第1a2期

机译：生物材料中砷的高精度放射化学中子活化分析，涉及通过混合和常规离子交换选择性分离砷
3. DEVELOPMENT AND TESTING OF A GREEN FLUORESCENT PROTEIN-BASED BACTERIAL BIOSENSOR FOR MEASURING BIOAVAILABLE ARSENIC IN CONTAMINATED GROUNDWATER SAMPLES [J] . Vivian Hsiu-Chuan Liao, Kun-Lin Ou Environmental toxicology and chemistry . 2005,第7期

机译：一种基于绿色荧光蛋白的细菌生物传感器的开发和测试，该传感器用于测定污染的地下水样品中的可生物利用的砷
4. COUPLED HPLC-ICP-MS ANALYSIS INDICATES HIGHLY HAZARDOUS CONCENTRATIONS OF DISSOLVED ARSENIC SPECIES IN CAMBODIAN GROUNDWATERS [C] . David A. Polya, Andrew G. Gault, Nicola J. Bourne, Plasma Source Mass Spectrometry, Applications and Emerging Technologies . 2003

机译：偶联HPLC-ICP-MS分析表明柬埔寨地下水中的溶解砷物种的高度危险浓度
5. Arsenic speciation analysis in environmental and food samples and investigation of sulfide in groundwater. [D] . He, Yi. 2005

机译：环境和食品样品中的砷形态分析以及地下水中的硫化物调查。
6. Rapid SPE – LC MS/MS analysis for atrazine its by-products simazine and S metolachlor in groundwater samples [O] . Hélène Blanchoud, Fabrice Alliot, Ningxin Chen, 2020

机译：快速SPE –地下水样品中阿特拉津其副产物Simazine和异丙甲草胺的LC MS / MS分析
7. The bacterial identification and antimicrobial susceptibility testing are usually performed manually. Recently, the number of samples has increased and the need for automatification has grown. BioMerieux has investigated Vitek2 for this purpose. HUSLAB has assigned us to compare the identification and antimicrobial results with respect to routine methods and Vitek2. Furthermore, we evaluate test reactions, ESBL (Extended Spectrum Beta-Lactamase) production and the time what it takes to get results with Vitek2. In this study, we examined a total of 183 bacterial isolates including 76 ESBL-strains, 40 nonfermenting rods, 41 fastidious bacteria and 26 fresh clinical isolates. The results of routine methods were gathered from the patient database and following this, compared to the results given by Vitek2. Vitek2 correctly identified more than 96 f the ESBL-strains and fresh clinical isolates. The results of nonfermenting rods and fastidious bacteri a were 70 oncordant with those of the routine methods. Test reaction results of Api20E were 94 nd Api20NE 70 n concordance with Vitek2 results. Approximately 80 f all Vitek2 antimicrobial results and 90 f ESBL test results were correct. Compared to earlier studies, Vitek2 performed the tests faster than the routine methods. These results suggest that Vitek2 is capable of giving rapid and accurate results. However, nonfermenting rods and fastidious bacteria should be studied further. [O] . Järveläinen Marika, Roslund Iira, Silander Maarit 2006

机译：细菌鉴定和抗菌药敏试验通常是手动进行的。最近，样品的数量增加了，并且对自动化的需求也在增长。 BioMerieux为此进行了Vitek2的研究。 HUSLAB已指派我们比较常规方法和Vitek2的鉴定和抗菌结果。此外，我们还评估了测试反应，ESBL（扩展频谱β-内酰胺酶）的产生以及使用Vitek2获得结果所需的时间。在这项研究中，我们检查了总共183个细菌分离株，包括76个ESBL菌株，40个非发酵棒，41个营养细菌和26个新鲜临床分离株。从患者数据库中收集常规方法的结果，然后将其与Vitek2给出的结果进行比较。 Vitek2正确鉴定出超过96种ESBL菌株和新鲜的临床分离株。与常规方法相比，非发酵棒和精制细菌的结果为70。 Api20E的测试反应结果与Vitek2结果一致为94nd Api20NE 70 n。所有Vitek2抗菌结果约80 f和ESBL测试结果90 f正确。与早期的研究相比，Vitek2的测试速度快于常规方法。这些结果表明Vitek2能够给出快速而准确的结果。但是，应进一步研究非发酵棒和营养细菌。

Bacterial bioassay for rapid and accurate analysis of arsenic in highly variable groundwater samples

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