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首页> 外文期刊>Environmental Science & Technology >Development of Estrogen-Responsive Transgenic Medaka for Environmental Monitoring of Endocrine Disrupters
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Development of Estrogen-Responsive Transgenic Medaka for Environmental Monitoring of Endocrine Disrupters

机译：内分泌干​​扰物环境监测的雌激素响应性转基因Medaka的开发。

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摘要

To develop a transgenic fish system to monitor environmental pollution,we generated a mvtg1:.gfp transgenic medaka line,in which the gfp reporter gene was under the control of medaka vitellogenin] (mvtg1) gene promoter.In this transgenic line,GFP was exclusively expressed in the liver of the mature adult female.Male and juvenile transgenic fish did not express GFP but could be induced to express GFP in the liver after exposure to 17-beta-estradiol (E2).Concurrent accumulation of mvtg1 and gfp mRNAs was observed during both development and estrogen treatment,indicating that the gfp transgene was faithfully expressed under the mvtg1 promoter.Dose- and time-dependent induction of GFP expression by E2 was investigated in male transgenic fish.The lowest-observed-effect concentration (LOEC) of E2 to induce GFP expression was 0.5 mug/L by observation of live fish and 0.05-0.1 mug/L by observation of dissection-exposed liver in a 30 day exposure experiment.GFP expression was observed within 36 h after treatment in high concentrations of E2 (5 mug/L),and it took longer to detect GFP expression under lower concentrations of E2.By removal and readdition of E2,we demonstrated that GFP expression was repeatedly induced.Finally,we also demonstrated that GFP expression could be induced by other estrogenic compounds,including 17-alpha-ethynylestradiol (EE2,0.05 mug/L),diethylstibestrol (DES,5 mug/L),estriol (10 mug/ L),and bisphenol A (BPA,1 mg/L),but not by weak estrogenic chemicals such as nonylphenol (NP,up to 1 mg/ L) and methoxychlor (MXC,up to 20 mug/L).Our experiments indicated the broad application of the transgenic line to monitor a wide range of estrogenic chemicals.
机译：为了开发监测环境污染的转基因鱼类系统，我们产生了一个mvtg1：.gfp转基因medaka品系，其中gfp报告基因在medaka vitellogenin]（mvtg1）基因启动子的控制下。在成年雌性的肝脏中表达GFP。公和少年转基因鱼暴露于17-β-雌二醇（E2）后不表达GFP，但可以诱导其在肝脏中表达GFP。观察到mvtg1和gfp mRNA的同时积累在雌激素处理过程中，表明gfp转基因在mvtg1启动子下忠实表达。研究了雄性转基因鱼中E2诱导的GFP剂量依赖性和时间依赖性。在30天的暴露实验中，通过观察活鱼观察到的E2诱导GFP表达为0.5杯/升，通过对解剖暴露的肝脏观察到的E2诱导GFP表达为0.05-0.1杯/升。在高浓度的E2（5杯/ L）处理后h，在较低浓度的E2下检测GFP表达需要花费更长的时间。通过去除和重新表达E2，我们证明了GFP表达被重复诱导。最后，我们还证明了GFP的表达可以被其他雌激素化合物诱导，包括17-α-乙炔基雌二醇（EE2,0.05杯/升），己烯雌酚（DES，5杯/升），雌三醇（10杯/升）和双酚A（BPA， 1 mg / L），但不受弱雌激素化学物质的影响，例如壬基酚（NP，最高1 mg / L）和甲氧基氯（MXC，最高20杯/ L）。我们的实验表明转基因品系在监测中的广泛应用多种雌激素化学物质。

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  • 来源
    《Environmental Science & Technology》 |2005年第22期|p.9001-9008|共8页
  • 作者

    ZHIQIANG ZENG; TAO SHAN; YAN TONG; SIEW HONG LAM; ZHIYUAN GONG;

  • 作者单位

    Department of Biological Sciences,National University of Singapore,Singapore;

    Department of Biological Sciences,National University of Singapore,Singapore;

    Department of Biological Sciences,National University of Singapore,Singapore;

    Department of Biological Sciences,National University of Singapore,Singapore;

    Department of Biological Sciences,National University of Singapore,Singapore;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
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