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首页> 外文期刊>Environmental Science & Technology >Evaluation of a Magnetic Particle-Based ELISA for the Determination of Chlorpyrifosethyl in Natural Waters and Soil Samples
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Evaluation of a Magnetic Particle-Based ELISA for the Determination of Chlorpyrifosethyl in Natural Waters and Soil Samples

机译：基于磁性粒子的ELISA测定天然水和土壤样品中毒死rif的评估

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摘要

A magnetic particle-based ELISA for chlorpyrifos-ethyl was investigated in estuarine water matrices, and the results obtained were correlated with automated on-line solid phase extraction (Prospekt) followed by liquid chromatography/diode array detection. The ELISA test was evaluated before and after filtration of the particulate matter present in estuarine water with and without prior acidification. The ELISA assay was used as a first screening method for determining chlorpyrifos-ethyl in a highly contaminated soil spiked at common termicidal application rate (～1000 mg/kg). The ELISA assay permitted us to distinguish soil contamination of chlorpyrifos-ethyl at 50 mg/kg in conjunction with Soxhlet extraction. Substantial saving time was achieved when compared to conventional methods, which require a cleanup step prior to chromatographic determinations.
机译：在河口水基质中对基于毒死rif的磁性ELISA进行了研究，所得结果与自动在线固相萃取（Prospekt），液相色谱/二极管阵列检测相关。在过滤和过滤之前和之后不进行酸化的情况下，对存在于河口水中的颗粒物进行过滤之前和之后对ELISA测试进行评估。 ELISA测定法是测定高污染土壤中常见杀虫剂施用量（〜1000 mg / kg）加标的毒死rif的第一种筛选方法。 ELISA测定法使我们能够与索氏提取法一起区分50 mg / kg的毒死rif乙基土壤污染。与传统方法相比，可节省大量时间，而传统方法在色谱测定之前需要清理步骤。

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