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Chlorine Penetration into Artificial Biofilm Is Limited by a Reaction—Diffusion Interaction

机译：氯渗透到人造生物膜中受反应-扩散相互作用的限制

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摘要

The retarded penetration of chlorine into artificial biofilms of Pseudomonas aeruginosa entrapped in agarose gel slabs was investigated experimentally and shown to be consistent with an unsteady reaction-diffusion model. A chlorine microelectrode was used to measure transient chlorine concentration profiles in artificial biofilms in a flow cell. While chlorine penetrated relatively quickly into pure agarose films (～15 min), its penetration into biofilms was greatly retarded when cells were present. The degree of retardation was proportional to the initial cell density in the biofilm. After 3 h of treatment with a flowing chlorine solution, the chlorine concentration at the substratum under a 526 μm thick biofilm containing 14 400 mg L~(-1) cell mass had only risen to 10% of the bulk solution value. A mathematical model of the transient reaction-diffusion interaction correctly captured the qualitative behavior of experimentally measured chlorine concentration profiles. Parameter values for the simulations were obtained from the literature and from independent investigations of bio-mass-chlorine reactions using well-mixed suspensions. Kinetic and stoichiometric coefficients for the reactions of agarose and cell mass with chlorine were obtained by fitting a simple first-order (in both reactants) kinetic model to chlorine versus time data. The reaction rate coefficient for chlorine-cell reaction (1.1 x 10~(-3) L mg~(-1) s~(-1)) exceeded that of chlorine-agarose reaction (3.7 x 10~(-6) L mg~(-1) s~(-1)) by 2 orders of magnitude. The yield coefficient relating the amount of cell mass consumed to the amount of chlorine consumed ranged from 0.6 to 4.3 mg mg~(-1), depending on the duration of the experiment. This study shows that the reaction rate of chlorine with cellular biomass is fast enough that diffusion of this disinfectant into the biofilm readily becomes rate limiting. This reaction—diffusion interaction affords an excellent explanation for the poor efficacy of chlorine when used against biofilm microorganisms.
机译：实验研究了氯在琼脂糖凝胶板上截留的对铜绿假单胞菌人工生物膜的阻滞渗透，并证明其与不稳定的反应扩散模型一致。氯微电极用于测量流通池中人造生物膜中的瞬时氯浓度分布。尽管氯相对较快地渗透到纯琼脂糖膜中（约15分钟），但是当存在细胞时，氯渗透到生物膜中的速度大大降低。延迟程度与生物膜中的初始细胞密度成正比。用流动的氯溶液处理3小时后，在526μm厚的生物膜下，含14400 mg L〜（-1）细胞质量的基质下的氯浓度仅上升到整体溶液值的10％。瞬态反应-扩散相互作用的数学模型正确地捕获了实验测得的氯浓度曲线的定性行为。模拟的参数值是从文献中以及使用充分混合的悬浮液进行的生物质-氯反应的独立研究中获得的。琼脂糖和细胞团与氯反应的动力学和化学计量系数是通过将简单的一阶（两种反应物）动力学模型拟合为氯随时间的数据而获得的。氯电池反应的反应速率系数（1.1 x 10〜（-3）L mg〜（-1）s〜（-1））超过氯琼脂糖反应的反应速率系数（3.7 x 10〜（-6）L mg 〜（-1）s〜（-1））2个数量级。取决于实验的持续时间，将消耗的细胞质量的量与消耗的氯的量相关联的屈服系数为0.6至4.3mg mg·（-1）。这项研究表明，氯与细胞生物质的反应速率足够快，以至于这种消毒剂向生物膜中的扩散很容易成为速率限制。这种反应-扩散相互作用为氯抗生物膜微生物的效果差提供了一个很好的解释。

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  • 来源
    《Environmental Science & Technology》 |1996年第6期|p.2078-2083|共6页
  • 作者

    XIAO CHEN; PHILIP S. STEWART;

  • 作者单位

    Center for Biofilm Engineering and Department of Chemical Engineering, Montana State University, Bozeman, Montana 59717;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 环境化学;
  • 关键词

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