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Impact of Physiological State on Surface Thermodynamics and Adhesion of Pseudomonas aeruginosa

机译：生理状态对铜绿假单胞菌表面热力学和粘附力的影响

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A quantitative understanding of microorganism migration in geological formations is critical to predict the dissemination of microorganisms in the environment and to evaluate the efficacy of microbially mediated in situ pollutant degradation. The key event that retards the movement of microorganisms in the saturated zone with respect to the convective water flow is the interaction between microorganisms and the matrix surfaces. This interaction may result in adhesion and concomitant retardation. Interactions are determined by the surface thermodynamics of the microorganism and the matrix. Whereas the nature of the matrix substratum surface may be considered temporally invariant, the nature of bacterial cell surfaces is a function of its physiological state. The work presented here explored quantitatively the impact of the physiological state of Pseudomonas aeruginosa Olin on its surface thermodynamic characteristics and its adhesion to dolomite. Lewis acid/ base (hydrophobic), Lifshitz-van der Waals (electrodynamic), and Coulombic (electrostatic) forces were measured via contact angle measurements and electrophoretic mobility assays. It was found that P. aeruginosa Olin exhibited a decreased electron-donating potential (γ_i~-) and increased ζ-potential in the stationary phase as compared with logarithmic growth and decay phases. These changes in surface thermodynamic properties were clearly manifested in subsequent partitioning experiments with dolomite. P. aeruginosa Olin was found to partition onto dolomite to a significantly larger extent in the stationary phase than in the logarithmic growth or decay phases. This observation further corroborates the need to include Lewis acid/base interactions in the evaluation of bacterium/surface interactions. The reported results indicate the clear impact of physiological state on surface thermodynamics and adhesion.
机译：对微生物在地质构造中迁移的定量了解对于预测微生物在环境中的传播以及评估微生物介导的原位污染物降解的有效性至关重要。相对于对流水流而言，阻碍微生物在饱和区运动的关键事件是微生物与基质表面之间的相互作用。这种相互作用可能导致粘附和随之而来的阻滞。相互作用取决于微生物和基质的表面热力学。尽管可以认为基质底层表面的性质在时间上是不变的，但是细菌细胞表面的性质是其生理状态的函数。本文介绍的工作定量地探讨了铜绿假单胞菌奥林的生理状态对其表面热力学特性及其与白云石的粘附性的影响。路易斯酸/碱（疏水），Lifshitz-van der Waals（电动）和库仑（静电）力通过接触角测量和电泳迁移率测定进行测量。结果发现，与对数生长期相比，铜绿假单胞菌奥林在固定相中的供电子势（γ_i〜-）降低，ζ电势升高。表面热力学性质的这些变化在随后的白云石分配实验中得到了明显体现。发现铜绿假单胞菌Olin在固定相中的分布程度要远大于对数生长期或衰减相时在白云岩上的分配。该观察结果进一步证实了在评估细菌/表面相互作用中需要包括路易斯酸/碱相互作用。报道的结果表明生理状态对表面热力学和粘附的明显影响。

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