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首页> 外文期刊>Environmental Science & Technology >Reductive Dehalogenation of Aliphatic Halocarbons by Lignin Peroxidase of Phanerochaete chrysosporium
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Reductive Dehalogenation of Aliphatic Halocarbons by Lignin Peroxidase of Phanerochaete chrysosporium

机译：Phanerochaete chrysosporium的木质素过氧化物酶对脂肪族卤代烃的还原脱卤作用

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摘要

Contamination of soils and aquifers by aliphatic halocarbons is a serious environmental pollution problem. We report here the novel observation that the halocarbons trichloroethylene (TCE) and CCl_4 were mineralized by Phanerochaete chrysosporium under aerobic conditions. Ligninolytic cultures of this white rot fungus mineralized 20.3% of 10 ppm TCE and 18.8% of 10 ppm CCl_4 in 9 days. These chemicals were not mineralized by nonligninolytic cultures of P. chrysosporium, indicating that lignin peroxidases play an important role in the mineralization of these chemicals. In a previous study, we reported lignin peroxidase-catalyzed reductive dehalogenation of CCl_4 with the resultantformation of trichloromethyl radical. We have extended this study and report here reductive dehalogenation of CHCl_3,CH_2Cl_2, TCE, and 1,1,1-trichloroethane. Dehalogenation was catalyzed by a reductive reaction system containing lignin peroxidase, veratryl alcohol, EDTA or oxalate, H_2O_2, and the halocarbon with phenyl N-tert-butylnitrone as a spin trap for electron spin resonance detection of the resulting radicals. Since all the components of the reductive system with oxalate as an electron donor are excreted by P. chrysosporium, we propose that this mechanism may be involved in the degradation of these halocarbons by the fungus.
机译：脂族卤代烃污染土壤和含水层是一个严重的环境污染问题。我们在这里报告了新颖的观察结果，卤化碳三氯乙烯（TCE）和CCl_4在好氧条件下被Phanerochaete chrysosporium矿化了。这种白腐真菌的木质素分解培养物在9天内矿化了20.3％的10 ppm TCE和18.8％的10 ppm CCl_4。这些化学物质没有被金孢假单胞菌的非木质素培养物矿化，表明木质素过氧化物酶在这些化学物质的矿化中起重要作用。在先前的研究中，我们报道了木质素过氧化物酶催化的CCl_4还原性脱卤化反应，并形成了三氯甲基自由基。我们扩展了这项研究，并在此报告了CHCl_3，CH_2Cl_2，TCE和1,1,1-三氯乙烷的还原性脱卤作用。通过包含木质素过氧化物酶，藜芦醇，EDTA或草酸酯，H_2O_2和卤代烃的还原反应体系催化脱卤，其中苯基N-叔丁基亚硝基作为自旋阱，用于电子自旋共振检测所得自由基。由于草酸作为电子给体的还原体系的所有成分都被金黄色葡萄球菌排泄，我们建议这种机制可能与真菌降解这些卤代烃有关。

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