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首页> 外文期刊>Environmental toxicology and pharmacology >Transcriptional regulation of glutathione biosynthesis genes, γ-glutamyl-cysteine ligase and glutathione synthetase in response to cadmium and nonylphenol in Chironomus riparius
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Transcriptional regulation of glutathione biosynthesis genes, γ-glutamyl-cysteine ligase and glutathione synthetase in response to cadmium and nonylphenol in Chironomus riparius

机译：谷氨酸对拟南芥中谷胱甘肽生物合成基因，γ-谷氨酰-半胱氨酸连接酶和谷胱甘肽合成酶的转录调控

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摘要

We characterized Chironomus riparius glutathione (GSH) biosynthesis genes, 7-glutamyl-cysteine ligase catalytic subunit (cr-gcl) and glutathione synthetase (cr-gs) and studied their expression after cadmium (Cd) and nonylphenol (NP) exposure. The full length cDNA of the Cr-GCL catalytic subunit was 2185 base pair (bp) in length containing an open reading frame of 1905 bp, a 13 bp 5' and 267 bp 3' untranslated regions. The theoretical molecular mass of the deduced amino acid sequence (633) was 72.65 kDa with an estimated pI of 5.42. The partial cDNA of Cr-GS was 739 bp in length consisting 221 amino acids. The deduced amino acid sequence of Cr-GCL and Cr-GS cDNAs showed high conservation with homologs from other species. In phylogenetic analysis Cr-GCL and Cr-GS were grouped with equivalent genes from insects belonging to the dipteran order. The expression of cr-gcl and cr-gs was measured using quantitative real-time PCR after exposure to sub lethal concentrations of Cd (2, 10 and 20 mg/L) and NP (10,50 and 100 μg/L) for 12,24,48 and 72 h using real-time PCR methods. The mRNA expression of Cr-GCL and Cr-GS was significantly modulated after exposure to different concentrations of Cd and NP for different time periods. Total GSH levels showed a non-significant decrease after exposure to Cd for 24 h. However, no change in GSH levels was observed after exposure to NP for 24 h. These results suggest that Cr-GS and Cr-GCL expression is modulated by Cd and NP stress and may play an important role in detoxification of xenobiotics and antioxidant defense. We conclude that Cr-GS and Cr-GCL could be used as biomarkers of Cd and NP stress in aquatic environment for the studied species.
机译：我们表征了河岸线虫谷胱甘肽（GSH）生物合成基因，7-谷氨酰-半胱氨酸连接酶催化亚基（cr-gcl）和谷胱甘肽合成酶（cr-gs）的特性，并研究了它们在镉（Cd）和壬基酚（NP）暴露后的表达。 Cr-GCL催化亚基的全长cDNA长度为2185个碱基对（bp），其中包含一个1905 bp的开放阅读框，一个13 bp的5'和267 bp的3'非翻译区。推导的氨基酸序列（633）的理论分子量为72.65 kDa，估计pI为5.42。 Cr-GS的部分cDNA长度为739 bp，包含221个氨基酸。推断的Cr-GCL和Cr-GS cDNA氨基酸序列与其他物种的同源物具有高度保守性。在系统发育分析中，将Cr-GCL和Cr-GS与来自昆虫的等价基因分组，这些昆虫属于二翅类。在暴露于亚致死浓度的Cd（2、10和20 mg / L）和NP（10,50和100μg/ L）12的致死浓度后，使用定量实时PCR测量cr-gcl和cr-gs的表达。 ，24、48和72小时使用实时PCR方法。 Cr-GCL和Cr-GS的mRNA表达在不同时间的不同浓度的Cd和NP下暴露后得到显着调节。接触镉24小时后，总谷胱甘肽水平没有明显下降。然而，暴露于NP 24小时后，未观察到GSH水平的变化。这些结果表明，Cr-GS和Cr-GCL的表达受Cd和NP胁迫的调节，可能在异生物素排毒和抗氧化防御中发挥重要作用。我们得出的结论是，Cr-GS和Cr-GCL可以作为所研究物种在水生环境中Cd和NP胁迫的生物标记。

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  • 来源
    《Environmental toxicology and pharmacology》 |2013年第2期|265-273|共9页
  • 作者

    Prakash M. Gopalakrishnan Nair; Sun Young Park; Ji Woong Chung; Jinhee Choi;

  • 作者单位

    School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea;

    School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea;

    School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea;

    School of Environmental Engineering, Graduate School of Energy and Environmental System Engineering, University of Seoul, 90 Jeonnong-dong, Dongdaemun-gu, Seoul 130-743, Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Chironomus riparius; Glutathione; γ-Glutamyl-cystein ligase; Glutathione synthetase; Cadmium; Nonylphenol;

    机译：希波罗努斯（Chironomus riparius）;谷胱甘肽;γ-谷氨酰半胱氨酸连接酶;谷胱甘肽合成酶;镉;壬基酚;

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