首页> 外文期刊>Environmental toxicology and chemistry >BIOACCUMULATION, STRESS, AND SWIMMING IMPAIRMENT IN DAPHNIA MAGNA EXPOSED TO MULTIWALLED CARBON NANOTUBES, GRAPHENE, AND GRAPHENE OXIDE
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BIOACCUMULATION, STRESS, AND SWIMMING IMPAIRMENT IN DAPHNIA MAGNA EXPOSED TO MULTIWALLED CARBON NANOTUBES, GRAPHENE, AND GRAPHENE OXIDE

机译:暴露于多壁碳纳米管,石墨烯和氧化石墨烯的水蚤中的生物累积,应力和游泳损伤

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The use of carbon-based nanomaterials (CNMs) such as multiwalled carbon nanotubes (MWCNTs), graphene, and graphene oxide (GO) is increasing across many applications because of their unique and versatile properties. These CNMs may enter the aquatic environment through many pathways, creating the potential for organism exposure. The present study addresses the bioaccumulation and toxicity seen in Daphnia magna exposed to CNMs dispersed in sodium dodecyl benzene sulfonate (SDBS). In study I, D. magna were exposed to varying outer diameters of MWCNTs for 24 h in moderately hard or hard freshwater. Bioaccumulation of MWCNT was found in all treatments, with the highest concentrations (0.53 +/- 0.27 mu g/g) in D. magna exposed in hard freshwater (p< 0.005). The median lethal concentration (LC50) was determined for D. magna exposed to CNMs in moderately hard and hard freshwater. In study II, D. magna were exposed to CNMs for 72 h in moderately hard freshwater to assess swimming velocity and generation of reactive oxygen species (ROS) detected by dichlorofluorescein fluorescence. An overall decrease was seen in D. magna swimming velocity after exposure to CNMs. The generation of ROS was significantly higher (1.54 +/- 0.38 dichlorofluorescein mM/mg dry wt) in D. magna exposed to MWCNTs of smaller outer diameters than in controls after 72 h (p<0.05). These results suggest that further investigation of CNM toxicity and behavior in the aquatic environment is needed. (C) 2017 SETAC.
机译:碳基纳米材料(CNM),例如多壁碳纳米管(MWCNT),石墨烯和氧化石墨烯(GO)的使用由于其独特而通用的性能而在许多应用中正在增加。这些CNM可能通过许多途径进入水生环境,从而有可能暴露生物。本研究针对暴露于分散在十二烷基苯磺酸钠(SDBS)中的CNM的大型蚤(Daphnia magna)中的生物蓄积性和毒性。在研究I中,D。magna在中等硬或硬的淡水中暴露于不同直径的MWCNTs中24小时。在所有处理中均发现了MWCNT的生物蓄积性,暴露于硬质淡水中的大果蝇中的最高浓度(0.53 +/- 0.27μg / g)(p <0.005)。测定了在中度硬和硬淡水中暴露于CNM的大果蝇的中值致死浓度(LC50)。在研究II中,将D. magna暴露于中度硬的淡水中的CNM 72小时,以评估游泳速度和通过二氯荧光素荧光检测的活性氧(ROS)的生成。接触CNM后,D。magna游泳速度总体下降。 72小时后,暴露于较小外径的MWCNT中的大果蝇中ROS的产生显着更高(1.54 +/- 0.38二氯荧光素mM / mg干重)(p <0.05)。这些结果表明,需要进一步研究CNM在水生环境中的毒性和行为。 (C)2017年SETAC。

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