...
首页> 外文期刊>Environmental toxicology and chemistry >ASSESSING BIOAVAILABILITY OF DDT AND METABOLITES IN MARINE SEDIMENTS USING SOLID-PHASE MICROEXTRACTION WITH PERFORMANCE REFERENCE COMPOUNDS
【24h】

ASSESSING BIOAVAILABILITY OF DDT AND METABOLITES IN MARINE SEDIMENTS USING SOLID-PHASE MICROEXTRACTION WITH PERFORMANCE REFERENCE COMPOUNDS

机译:固相微萃取与性能参考化合物评估海洋沉积物中DDT和代谢产物的生物利用度

获取原文
获取原文并翻译 | 示例
           

摘要

Solid-phase microextraction (SPME) has often been used to estimate the freely dissolved concentration (C_(free)) of organic contaminants in sediments. A significant limitation in the application of SPME for C_(free) measurement is the requirement for attaining equilibrium partition, which is often difficult for strongly hydrophobic compounds such as DDT. A method was developed using SPME with stable isotope-labeled analogues as performance reference compounds (PRCs) to measure C_(free) of DDT and metabolites (DDTs) in marine sediments. Six ~(13)C-labeled or deuterated PRCs were impregnated into polydimethylsiloxane (PDMS) fiber before use. Desorption of PRCs from PDMS fibers and absorption of DDTs from sediment were isotropic in a range of sediments evaluated ex situ under well-mixed conditions. When applied to a historically contaminated marine sediment from a Superfund site, the PRC-SPME method yielded C_(free) values identical to those found by using a conventional equilibrium SPME approach (Eq-SPME), wherease the time for mixing was reduced from 9 d to only 9 h. The PRC-SPME method was further evaluated against bioaccumulation of DDTs by Neanthes arenaceodentata in the contaminated sediment with or without amendment of activated carbon or sand. Strong correlations were consistently found between the derived equilibrium concentrations on the fiber and lipid-normalized tissue residues for DDTs in the worms. Results from the present study clearly demonstrated the feasibility of coupling PRCs with SPME sampling to greatly shorten sampling time, thus affording much improved flexibility in the use of SPME for bioavailability evaluation.
机译:固相微萃取(SPME)通常用于估算沉积物中有机污染物的自由溶解浓度​​(C_(free))。 SPME在C_(free)测量中应用的一个显着局限是需要达到平衡分配,这对于强疏水性化合物(如DDT)通常是困难的。使用SPME开发了一种方法,该方法使用稳定的同位素标记的类似物作为性能参考化合物(PRC)来测量海洋沉积物中DDT和代谢物(DDT)的C_(游离)。在使用前,将六个〜(13)C标记或氘代的PRCs浸渍到聚二甲基硅氧烷(PDMS)纤维中。在充分混合的条件下,就地评估了一系列沉积物中,PDMS从PDMS纤维上的解吸和DDT从沉积物中的吸收是各向同性的。当将PRSP-SPME方法应用于来自超级基金站点的历史受污染的海洋沉积物时,其C_(free)值与使用常规平衡SPME方法(Eq-SPME)得出的值相同,因此混合时间从9减少了d仅9小时。进一步评估了PRC-SPME方法在被污染的沉积物中添加或不添加活性炭或沙的情况下,对被污染的泥沙中的滴滴涕的生物积累的影响。在蠕虫中DDT的纤维上的平衡浓度与脂质归一化的组织残留之间始终存在强相关性。本研究的结果清楚地表明了将PRCs与SPME采样结合以大大缩短采样时间的可行性,从而在使用SPME进行生物利用度评估时提供了更大的灵活性。

著录项

  • 来源
    《Environmental toxicology and chemistry》 |2013年第9期|1946-1953|共8页
  • 作者单位

    Department of Environmental Science, University of California, Riverside, California, USA,State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China;

    Department of Environmental Science, University of California, Riverside, California, USA;

    Department of Environmental Science, University of California, Riverside, California, USA;

    State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China;

    Department of Environmental Science, University of California, Riverside, California, USA;

    Department of Environmental Science, University of California, Riverside, California, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Solid-phase microextraction (SPME); Bioavailability; Performance reference compound; Passive sampling; DDT;

    机译:固相微萃取(SPME);生物利用度;性能参考化合物;被动采样;滴滴涕;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号