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首页> 外文期刊>Environmental toxicology and chemistry >RELATIONSHIPS BETWEEN SURFACE-BOUND AND INTERNALIZED COPPER AND CADMIUM AND TOXICITY IN CHLAMYDOMONAS REINHARDTII
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RELATIONSHIPS BETWEEN SURFACE-BOUND AND INTERNALIZED COPPER AND CADMIUM AND TOXICITY IN CHLAMYDOMONAS REINHARDTII

机译：雷氏菌表面结合铜和离子化铜与镉的关系和毒性

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摘要

In the present study, the adsorption and uptake of copper (Cu) and cadmium (Cd) in Chlamydomonus reinhardtii were examined to establish fundamental toxicity relationships to glutathione and cell-growth endpoints. Establishing these fundamental relationships of metal accumulation and toxicity metrics is necessary to subsequently implement an algal biotic ligand model. The glutathione response was similar to the response measured from growth endpoints for both internal and adsorbed Cu, indicating that glutathione may be a useful biomarker of toxicity. The glutaihione response with Cd contrasted markedly with that observed with Cu and was therefore observed to be a metal-specific biomarker. The density of sites binding metals and the related stability constants for the algal cell surface were also determined. Short exposures to metals (2h) were conducted, and we determined 6.0 × 10~(-6)mol/g sites binding Cu and 2.0 × 10~(-6)mol/g sites binding Cd and conditional stability constants as log K' = 1.2 and log K' = 6.7 for Cu and Cd, respectively. Experiments were also conducted to determine the effect on toxicity endpoints of varying nitrate concentrations and different humic acids (HA) in the exposure media. Varying nitrate concentrations did not have an effect on cell growth over 24 h. The surface-adsorbed Cu measurements from the experiments with HA depended on the type and concentration of HA.
机译：在本研究中，研究了莱茵衣藻中铜（Cu）和镉（Cd）的吸附和吸收，以建立与谷胱甘肽和细胞生长终点的基本毒性关系。建立金属积累和毒性指标的这些基本关系对于随后实施藻类生物配体模型是必要的。谷胱甘肽反应类似于从生长终点对内部和吸附的铜的反应，表明谷胱甘肽可能是有用的毒性生物标志物。谷氨酸对镉的响应与铜的响应明显不同，因此被认为是金属特异性的生物标志物。还确定了结合金属的位点的密度以及藻细胞表面的相关稳定性常数。短时间暴露于金属（2h），我们确定结合Cu的6.0×10〜（-6）mol / g位点和结合Cd的2.0×10〜（-6）mol / g位点和条件稳定性常数作为log K' Cu和Cd分别为1.2和log K'= 6.7。还进行了实验以确定暴露介质中硝酸盐浓度和腐殖酸（HA）变化对毒性终点的影响。硝酸盐浓度的变化在24小时内对细胞生长没有影响。 HA实验中表面吸附的Cu值取决于HA的类型和浓度。

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