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首页> 外文期刊>Environmental toxicology and chemistry >PHYTOTOXKIT: A CRITICAL LOOK AT A RAPID ASSESSMENT TOOL
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PHYTOTOXKIT: A CRITICAL LOOK AT A RAPID ASSESSMENT TOOL

机译：PHYTOTOXKIT：快速评估工具的关键看

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摘要

Terrestrial plant toxicity testing contributes critical information to many site risk assessments, but standardized tests can be labor-intensive, use large amounts of soil, and employ long test durations. The Phytotoxkit (MicroBioTests, Environmental Bio-Detection Products) minimizes the time and cost associated with terrestrial plant testing with a unique test setup, a shorter test duration, and less soil. However, the sensitivity of the test remains an open question. In this research, the Phytotoxkit and the standardized Environment Canada terrestrial plant toxicity test (definitive test) are compared using a parallel testing approach. Three different scenarios were examined: a multiconcentration test, in which an inhibiting concentration (ICp) was derived from chemically amended soils; a soil remediation test, in which plant growth in a remediated soil was compared to the original contaminated soil; and a site soil test, in which plant growth in a contaminated soil was compared to a reference soil. The contaminants tested were boric acid, Cr(VI) with cyclodextrin as a remediation agent, and petroleum hydrocarbons. Trifolium pratense (red clover) was used in the first and second scenarios, and six different plant species were used in the third scenario. In the first scenario, the Phytotoxkit results compared well with the definitive test results after 5 and 7 d of exposure. In the second scenario, the Phytotoxkit results agreed with the definitive test when evaluating the effectiveness of remediation. In the third scenario, the Phytotoxkit results were often not in agreement with the results from the definitive test. The reduced sensitivity of the Phytotoxkit in the third scenario may be driven by test unit design, as plant roots are separated from soil by filter paper.
机译：陆生植物毒性测试为许多现场风险评估提供了重要信息，但是标准化测试可能需要大量劳动，占用大量土壤并且需要较长的测试时间。 Phytotoxkit（MicroBioTests，环境生物检测产品）通过独特的测试设置，更短的测试时间和更少的土壤，最大程度地减少了与陆地植物测试相关的时间和成本。但是，测试的敏感性仍然是一个悬而未决的问题。在这项研究中，使用并行测试方法比较了Phytoxkit和加拿大环境部陆地植物毒性测试（确定性测试）。研究了三种不同的情况：多浓度试验，其中化学修饰土壤产生抑制浓度（ICp）；土壤修复试验，将修复土壤中的植物生长与原始污染土壤进行比较；现场土壤测试，将受污染土壤中的植物生长与参考土壤进行比较。测试的污染物是硼酸，以环糊精为媒介的Cr（VI）和石油烃。在第一种和第二种情况下使用了三叶草（红三叶草），在第三种情况下使用了六种不同的植物物种。在第一种情况下，Phytoxkit的结果与暴露5天和7天后的确定测试结果进行了很好的比较。在第二种情况下，Phytoxkit的结果与评估修复效果的最终测试结果一致。在第三种情况下，Phytotokit的结果通常与确定性测试的结果不一致。第三种情况下Phytotoxkit灵敏度降低的原因可能是测试单元的设计，因为植物的根部通过滤纸与土壤分开。

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  • 来源
    《Environmental toxicology and chemistry》 |2012年第2期|p.316-323|共8页
  • 作者

    Leana Van der Vliet; Jessica Velicogna; Juliska Princz; Richard Scroggins;

  • 作者单位

    Biological Assessment and Standardization Section, Environment Canada, Ottawa, Ontario, Canada;

    Science Applications International Corporation Canada, Ottawa, Ontario, Canada;

    Biological Assessment and Standardization Section, Environment Canada, Ottawa, Ontario, Canada;

    Biological Assessment and Standardization Section, Environment Canada, Ottawa, Ontario, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bioassay; rapid assessment; phytotoxkit; soil ecotoxicity; power analysis;

    机译：生物测定快速评估;phytotoxkit;土壤生态毒性;功率分析;

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