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首页> 外文期刊>Environmental toxicology and chemistry >RESPONSES OF MICROBIAL COMMUNITY FROM NORTHERN GULF OF MEXICO SANDY SEDIMENTS FOLLOWING EXPOSURE TO DEEPWATER HORIZON CRUDE OIL
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RESPONSES OF MICROBIAL COMMUNITY FROM NORTHERN GULF OF MEXICO SANDY SEDIMENTS FOLLOWING EXPOSURE TO DEEPWATER HORIZON CRUDE OIL

机译:暴露于深层原油后,墨西哥沙质沉积物北部湾微生物群落的响应

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摘要

In the present study, microbial community responses to exposure to unweathered Macondo Well crude oil and conventional diesel in a sandy beach environment were determined. Biodegradation was assessed in mesocosm experiments with differing fuel amounts (2,000 and 4,000 mg/kg) and with or without inorganic nutrient amendment. Carbon dioxide production was measured daily for 42 d. Aerobic alkane, total hydrocarbon, and polycyclic aromatic hydrocarbon (PAH) degraders were enumerated in treated and control mesocosms and changes in their abundances were measured weekly. Hydrocarbon mineralization occurred in all treatments. In the inorganic nutrient-amended treatments, the degradation rates were 2.31 and 2.00 times greater in the 2,000 mg/kg diesel and crude oil treatments, respectively, and 3.52 (diesel) and 4.14 (crude) times higher for the fuel types at the 4,000 mg/kg fuel concentrations compared to unamended treatments. Microbial lag phases were short (<3d) and alkane and total hydrocarbon degrader numbers increased by five orders of magnitude compared to the uncontaminated treatments within 7 d in most treatments. Hydrocarbon degrader numbers in diesel and in crude oil treatments were similar; however, the PAH degraders were more abundant in the crude oil relative to diesel treatment. These findings indicate that hydrocarbon degradation by extant microbial populations in the northern Gulf of Mexico sandy beach environments can be stimulated and enhanced by inorganic nutrient addition.
机译:在本研究中,确定了微生物群落对在沙滩环境中暴露于未风化的Macondo井原油和常规柴油的反应。在介观实验中评估了生物降解情况,其中燃料用量不同(2,000和4,000 mg / kg),有或没有无机营养改良剂。每天测量42天的二氧化碳产量。列举了好氧烷烃,总烃和多环芳烃(PAH)的降解物,并对其进行了每周处理。所有处理均发生烃矿化。在无机养分改良处理中,在2,000 mg / kg柴油和原油处理中,降解率分别高出2.31和2.00倍,在4,000时,燃料类型的降解率分别高出3.52(柴油)和4.14(粗)倍。毫克/千克燃料浓度与未经修正的处理相比。在大多数处理中,微生物滞后期较短(<3天),与未污染处理相比,在7天内,烷烃和总烃降解物数量增加了五个数量级。柴油和原油处理中的烃降解剂数量相似。然而,相对于柴油处理,PAH降解剂在原油中含量更高。这些发现表明,在墨西哥湾北部沙滩环境中,现有微生物种群对烃类的降解可以通过添加无机营养物来促进和增强。

著录项

  • 来源
    《Environmental toxicology and chemistry》 |2012年第5期|p.1004-1011|共8页
  • 作者单位

    Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama, USA Dauphin Island Sea Lab, Dauphin Island, Alabama, USA;

    Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama, USA Dauphin Island Sea Lab, Dauphin Island, Alabama, USA;

    Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biodegradation; crude oil; deepwater horizon; oil spill; macondo;

    机译:生物降解原油;深水地平线漏油事件;马通多;

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