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首页> 外文期刊>Environmental toxicology and chemistry >mRNA EXPRESSION AND ACTIVITY OF ION-TRANSPORTING PROTEINS IN GILLS OF THE BLUE CRAB CALLINECTES SAPIDUS: EFFECTS OF WATERBORNE COPPER
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mRNA EXPRESSION AND ACTIVITY OF ION-TRANSPORTING PROTEINS IN GILLS OF THE BLUE CRAB CALLINECTES SAPIDUS: EFFECTS OF WATERBORNE COPPER

机译：蓝蟹CALIDCTES SAPIDUS G中mRNA的表达和离子运输蛋白的活性：水性铜的影响

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摘要

Waterborne Cu effects on the transcription of genes encoding ion-transporting proteins and the activities of these proteins were evaluated in gills of the blue crab Callinectes sapidus acclimated to diluted (2%o) and full (30%o) seawater. Crabs were exposed (96 h) to an environmentally relevant concentration of dissolved Cu (0.78 μM) and had their posterior (osmoregulating) gills dissected for enzymatic and molecular analysis. Endpoints analyzed were the activity of key enzymes involved in crab osmoregulation (sodium-potassium adenosine triphosphatase [Na~+/K~+-ATPase], hydrogen adenosine triphosphatase [H~+-ATPase], and carbonic anhydrase [CA]) and the mRNA expression of genes encoding these enzymes and the sodium-potassium-chloride (Na~+/K~+/2C1~ -) cotransporter. Copper effects were observed only in crabs acclimated to diluted seawater (hyperosmoregulating crabs) and were associated with an inhibition of the expression of mRNA of genes encoding the Na~+/K~+-ATPase and the Na~+/K~+/2C1~ - cotransporter. However, Cu did not affect Na~+/K~+-ATPase activity, indicating that the gene transcription is downregulated before a significant inhibition of the enzyme activity can be observed. This also suggests the existence of a compensatory response of this enzyme to prevent osmoregulatory disturbances after short-term exposure to environmentally relevant Cu concentrations. These findings suggest that Cu is a potential ionoregulatory toxicant in blue crabs C. sapidus acclimated to low salinity. The lack of Cu effect on blue crabs acclimated to full seawater would be due to the reduced ion uptake needed for the regulation of the hemolymph osmotic concentration in full seawater (30‰). Also, this could be explained considering the lower bioavailability of toxic Cu (free ion) associated with the higher ionic content and dissolved organic matter concentration in high salinity (30‰) than in diluted seawater (2 ‰).
机译：在适应稀释的（2％o）和完全的（30％o）海水的蓝蟹Call（Callinectes sapidus）evaluated中，评估了水铜对编码离子转运蛋白的基因转录和这些蛋白活性的影响。将螃蟹暴露于环境相关浓度的溶解铜（0.78μM）中（96 h），并将其后（渗透压）more切开，以进行酶和分子分析。分析的终点为参与蟹渗透压调节的关键酶的活性（钠钾腺苷三磷酸酶[Na〜+ / K〜+ -ATPase]，氢腺苷三磷酸酶[H〜+ -ATPase]和碳酸酐酶[CA]）和编码这些酶和氯化钠-钾-钾（Na〜+ / K〜+ / 2C1-）的基因的mRNA表达。仅在适应稀释海水的螃蟹（高渗调节性螃蟹）中观察到铜效应，并与抑制Na〜+ / K〜+ -ATPase和Na〜+ / K〜+ / 2C1编码基因的mRNA表达有关〜-共同运输者。然而，Cu不会影响Na〜+ / K〜+ -ATPase的活性，表明在观察到对酶活性的显着抑制之前，基因转录被下调。这也表明该酶存在补偿反应，以防止短期暴露于环境相关的铜浓度后引起的渗透调节紊乱。这些发现表明，铜是适应低盐度的蓝蟹C. sapidus的潜在离子调节毒物。铜不能使蓝蟹适应全海水，这是由于调节全海水中的血淋巴渗透浓度所需的离子吸收减少（30‰）。另外，考虑到高盐度（30‰）比稀释海水（2‰）中较高的离子含量和溶解的有机物浓度，有毒的Cu（游离离子）的生物利用度较低，可以解释这一点。

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  • 来源
    《Environmental toxicology and chemistry》 |2011年第1期|p.206-211|共6页
  • 作者

    Camila M. G. Martins; Daniela Volcan Almeida; Luis Fernando Fernandes Marins; Adalto Bianchini;

  • 作者单位

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), 96201-900 Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), 96201-900 Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), 96201-900 Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande (FURG), 96201-900 Rio Grande, Rio Grande do Sul, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    crab; copper; mRNA expression; ion transporters; salinity;

    机译：螃蟹;铜;mRNA表达;离子转运子盐度;

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