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首页> 外文期刊>Environmental toxicology and chemistry >SCREENING COMPLEX EFFLUENTS FOR ESTROGENIC ACTIVITY WITH THE T47D-KBLUC CELL BIOASSAY: ASSAY OPTIMIZATION AND COMPARISON WITH IN VIVO RESPONSES IN FISH
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SCREENING COMPLEX EFFLUENTS FOR ESTROGENIC ACTIVITY WITH THE T47D-KBLUC CELL BIOASSAY: ASSAY OPTIMIZATION AND COMPARISON WITH IN VIVO RESPONSES IN FISH

机译：T47D-KBLUC细胞生物测定筛查雌激素活性的复杂流出物：鱼中活体反应的测定优化和比较

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摘要

Wastewater treatment plant (WWTP) effluents can contain estrogenic chemicals, which potentially disrupt fish reproduction and development. The current study focused on the use of an estrogen-responsive in vitro cell bioassay (T47D-KBluc), to quantify total estrogenicity of WWTP effluents. We tested a novel sample preparation method for the T47D-KBluc assay, using powdered media prepared with direct effluent. Results of the T47D-KBluc assay were compared with the induction of estrogen receptor-regulated gene transcription in male fathead minnows (Pimephales promelas) exposed to the same effluents. Effluent samples for the paired studies were collected over the course of three months. According to the T47D-KBluc assay, the effluent estrogenicity ranged from 1.13 to 2.00 ng 17β-estradiol (E2) equivalents/L. Corresponding in vivo studies exposing male fathead minnows to 0, 10, 50, and 100% effluent dilutions demonstrated that exposure to 100% effluent significantly increased hepatic vitellogenin (VTG) and estrogen receptor α subunit transcripts relative to controls. The induction was also significant in males exposed to 250 ng E2/L or 100 ng E2/L. The in vitro and in vivo results support the conclusion that the effluent contains significant estrogenic activity, but there was a discrepancy between in vitro- and in vivo-based E2 equivalent estimates. Our results suggest that the direct effluent preparation method for the T47D-KBluc assay is a reasonable approach to estimate the estrogenicity of wastewater effluent.
机译：废水处理厂（WWTP）的废水中可能含有雌激素化学物质，可能会破坏鱼类的繁殖和发育。当前的研究集中在使用雌激素反应性体外细胞生物测定法（T47D-KBluc），以量化WWTP废水的总雌激素性。我们使用直接流出物制备的粉末状介质测试了T47D-KBluc测定的新型样品制备方法。将T47D-KBluc分析的结果与暴露于相同流出液的雄性黑头min鱼（Pimephales promelas）中雌激素受体调节的基因转录的诱导进行了比较。在三个月的过程中收集了用于配对研究的污水样品。根据T47D-KBluc分析，流出物的雌激素性范围为1.13至2.00 ng17β-雌二醇（E2）当量/ L。相应的体内研究显示，将雄性黑头min鱼暴露于0％，10％，50％和100％的废水稀释液中表明，相对于对照，暴露于100％的废水显着增加了肝卵黄蛋白原（VTG）和雌激素受体α亚基的转录物。在暴露于250 ng E2 / L或100 ng E2 / L的雄性中，诱导也很明显。体外和体内结果支持这样的结论，即流出物含有明显的雌激素活性，但基于体外和体内的E2当量估算值之间存在差异。我们的结果表明，用于T47D-KBluc测定的直接废水制备方法是估算废水流出物雌激素性的合理方法。

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  • 来源
    《Environmental toxicology and chemistry》 |2011年第2期|p.439-445|共7页
  • 作者

    Leah C. Wehmas; Jenna E. Cavallin; Elizabeth J. Durhan; Michael D. Kahl; Dalma Martinovic; Joe Mayasich; Tim Tuominen; Daniel L. Villeneuve; Gerald T. Ankley;

  • 作者单位

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota,University of St. Thomas, St. Paul, Minnesota, USA;

    Western Lake Superior Sanitary District, Duluth, Minnesota, USA;

    Western Lake Superior Sanitary District, Duluth, Minnesota, USA;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

    U.S. Environmental Protection Agency, Office of Research and Development, Duluth, Minnesota;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    estrogens; gene expression; complex mixtures; solid-phase extraction;

    机译：雌激素基因表达;复杂混合物固相萃取;

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