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首页> 外文期刊>Environmental toxicology and chemistry >EFFECT OF COPPER ON ION CONTENT IN ISOLATED MANTLE CELLS OF THE MARINE CLAM MESODESMA MACTROIDES
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EFFECT OF COPPER ON ION CONTENT IN ISOLATED MANTLE CELLS OF THE MARINE CLAM MESODESMA MACTROIDES

机译：铜对海洋生物大麦中线粒体离体上皮细胞离子含量的影响

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摘要

The effect of copper on ion content (Na~+, K~+, Ca~(2+), and Cl~-) was evaluated in isolated mantle gills of the marine clam Mesodesma mactroides. Clams were collected at the Mar Grosso Beach (Sao Jose do Norte, Rio Grande do Sul [RS], southern Brazil), cryoanesthetized, and had their mantles dissected. Mantle cells were isolated and incubated in a calcium-free phosphate solution without (control) or with Cu (CuCl_2). Cells were exposed to Cu for 1 h (5 μM) or 3 h (2.5 and 5 μM). In cells incubated with 2.5 |jiM Cu, a significant decrease in intracellular Cl~ content was observed. However, in cells incubated with 5.0 μM Cu, significant reductions in Na~+, K~+, and Cl~- intracellular content were observed. Given the mechanisms involved in ion transport in mantle cells of the marine clam M. mactroides, the findings described here suggest that Cu exposure inhibits carbonic anhydrase and Na~+/K~+-ATPase activity. Also, it can be suggested that Cu is competing with Na~+ for the same mechanisms of ion transport in the cell membrane, such as the Na~+ channels and the Na~+/K~+/2C1~ cotransporter". Results from the present study also clearly indicate that processes involved in cellular anion regulation are more sensitive to Cu exposure than those associated with the cellular cation regulation. Characterization of sites for Cu accumulation and toxicity in aquatic animals is important for derivation of metal binding constants at the biotic ligand. Also, identification of the mechanism of metal toxicity is needed for modeling metal accumulation in the biotic ligand and its consequent toxicity. Therefore, the findings reported here are extremely valuable for the development of a biotic ligand model version for marine and estuarine waters.
机译：在海洋蛤mac中线分离的地幔g中评估了铜对离子含量（Na〜+，K〜+，Ca〜（2+）和Cl〜-）的影响。在Mar Grosso海滩（巴西南部南里奥格兰德州圣保罗，南圣何塞）采集蛤,，进行冷冻麻醉，并解剖其地幔。分离套细胞并在不含钙的磷酸钙溶液中（对照）或与铜（CuCl_2）一起孵育。将细胞暴露于Cu 1 h（5μM）或3 h（2.5和5μM）。在用2.5μMCu孵育的细胞中，观察到细胞内Cl〜含量明显降低。然而，在用5.0μMCu孵育的细胞中，观察到Na〜+，K〜+和Cl〜-细胞内含量的显着降低。考虑到涉及到海洋蛤仔M.壳层细胞中离子迁移的机制，这里描述的发现表明Cu暴露抑制了碳酸酐酶和Na〜+ / K〜+ -ATPase的活性。而且，可以暗示Cu与Na〜+竞争细胞膜中离子迁移的相同机制，例如Na〜+通道和Na〜+ / K〜+ / 2C1〜共转运蛋白。”本研究还清楚地表明，与细胞阳离子调节相关的过程相比，参与细胞阴离子调节的过程对铜的暴露更为敏感，对水生动物体内铜积累和毒性位点的表征对于生物体中金属结合常数的推导很重要。同样，需要鉴定金属毒性的机理来模拟生物在生物配体中的蓄积及其随后的毒性，因此，本文报道的发现对于开发用于海洋和河口水的生物配体模型非常有价值。

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  • 来源
    《Environmental toxicology and chemistry》 |2011年第7期|p.1582-1585|共4页
  • 作者

    Thais Martins Lopes; Indianara Fernanda Barcarolli; Camila Bento de Oliveira; Marta Marques de Souza; Adalto Bianchini;

  • 作者单位

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil;

    Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande, Rio Grande, Rio Grande do Sul, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    clam; copper; ion content; mantle cells; mesodesma mactroides;

    机译：蛤;铜;离子含量幔细胞中线;

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