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首页> 外文期刊>Environmental toxicology and chemistry >COMPARATIVE HEPATIC MICROSOMAL BIOTRANSFORMATION OF SELECTED PBDES, INCLUDING DECABROMODIPHENYL ETHER, AND DECABROMODIPHENYL ETHANE FLAME RETARD ANTS IN ARCTIC MARINE-FEEDING MAMMALS
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COMPARATIVE HEPATIC MICROSOMAL BIOTRANSFORMATION OF SELECTED PBDES, INCLUDING DECABROMODIPHENYL ETHER, AND DECABROMODIPHENYL ETHANE FLAME RETARD ANTS IN ARCTIC MARINE-FEEDING MAMMALS

机译:北极海洋哺乳类哺乳动物中选定PBDES的比较性肝微生物生物转化,包括十溴二苯醚和十溴二苯甲烷火焰缓释蚂蚁

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摘要

The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209.
机译:本研究评估和比较了溴化阻燃剂的氧化和还原生物转化,包括建立的多溴化二苯醚(PBDEs)和新兴的十溴二苯乙烷(DBDPE),使用了基于北极北极海洋饲养哺乳动物肝脏微粒体的体外系统。 (Ursus maritimus),白鲸(Delphinapterus leucas)和环斑海豹(Pusa hispida),并在实验室大鼠中作为哺乳动物的模型物种。与较低溴化PBDEs(BDE 99,100和154; 30pmol的0-3%)的消耗相比,所有物种的个体中全溴化BDE209(30pmol的14-25%)和DBDPE(90pmol的44-74%)的消耗更大。 )。没有观察到简单脱溴代谢物的证据。对大鼠和北极熊中的酚类代谢产物的研究表明,北极熊中形成了两种酚类,可能是多溴化的溴化DBDPE代谢物,北极熊和大鼠微粒体中形成了一种酚类BDE154代谢物。对于BDE209和DBDPE,尽管母体大量耗竭,但观察到的代谢物浓度仍低至无法检测。这些发现提示可能低估了总BDE209及其转化产物的生态系统负担,并且需要进行研究以鉴定和表征主要BDE209代谢物的持久性和毒性。考虑到理化性质和环境行为与BDE209相似,当前生物转化的证据以及DBDPE替代BDE209的使用增加,DBDPE可能存在类似的问题。

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