...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >THE FEASIBILITY OF USING MOSQUITOFISH (GAMBUSIA AFFINIS) FOR DETECTING ENDOCRINE-DISRUPTING CHEMICALS IN THE FRESHWATER ENVIRONMENT
【24h】

THE FEASIBILITY OF USING MOSQUITOFISH (GAMBUSIA AFFINIS) FOR DETECTING ENDOCRINE-DISRUPTING CHEMICALS IN THE FRESHWATER ENVIRONMENT

机译：用蚊虫检测淡水环境中破坏内分泌的化学药品的可行性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We evaluated the utility of gene-transcriptional responses in the liver of mosquitofish (Gambusia affinis), a species introduced to many countries and therefore widely available, for detecting endocrine-disrupting activity in water. Exposure to β-naphthoflavone, an aryl hydrocarbon receptor (AhR) agonist, significantly increased the transcript of the cytochrome P4501A gene (cypla), peaking at 24 h, in both sexes at concentrations of 10 μg/L or more. 17β-Estradiol (E_2) at 500ng/L increased the number of males showing gene transcription of precursors of yolk protein, vitellogenin (Vtga, Vtgb, and Vtgc), at 24, 48, and 72 h. Exposure for 48 h to bisphenol A (BPA), an estrogen mimic, also increased vtg-positive males at 1 mg/L or more. Leachate from a Japanese stable-type landfill significantly increased vtg-positive males after 48 h exposure, and the in vitro activity of the leachate against the estrogen receptor (ER) was estimated as an E_2 equivalent of 240 ng/L by yeast transfected with the ER. Chemical analysis showed that major contributors to the ER activation were BPA and 4-tert-octylphenol. This leachate and drainage water from a control-type landfill had AhR activities, estimated by yeast with the AhR, but had no significant effect on cypla transcription. These results showed that mosquitofish are suitable for detecting in vivo AhR and ER effects, but are insensitive to E_2.
机译：我们评估了蚊子（Gambusia affinis）肝脏中的基因转录反应的效用，该物种已被引入许多国家，因此广泛用于检测水中的内分泌干扰活性。暴露于浓度为10μg/ L或更高的男女中，暴露于芳烃受体（AhR）激动剂β-萘黄酮的细胞色素P4501A基因（cypla）的转录本均在24小时达到峰值。在24、48和72小时时，浓度为500ng / L的17β-雌二醇（E_2）增加了显示卵黄蛋白，卵黄蛋白原（Vtga，Vtgb和Vtgc）前体基因转录的雄性数量。雌激素模拟物双酚A（BPA）暴露48 h也增加了1 mg / L或更高的vtg阳性雄性。来自日本稳定型垃圾填埋场的渗滤液在暴露48小时后显着增加了vtg阳性的雄性，转染酵母的渗滤液对雌激素受体（ER）的体外活性估计为E_2当量，为240 ng / L。 ER。化学分析表明，ER活化的主要贡献者是BPA和4-叔辛基苯酚。对照型垃圾填埋场的渗滤液和排水具有AhR活性，这是酵母与AhR估计的，但对Cypla转录没有明显影响。这些结果表明蚊鱼适合检测体内的AhR和ER效应，但对E_2不敏感。

著录项

  • 来源
    《Environmental toxicology and chemistry》 |2011年第12期|p.2778-2785|共8页
  • 作者

    Ryo Kamata; Keisuke Itoh; Daisuke Nakajima; Shiho Kageyama; Akiyoshi Sawabe; Masanori Terasaki; Fujio Shirashi;

  • 作者单位

    Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan;

    Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan;

    Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan;

    Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan;

    Faculty of Agriculture, Kinki University, Nara, Nara, Japan;

    Institute for Environmental Sciences, University of Shizuoka, Suruga-ku, Shizuoka, Shizuoka, Japan;

    Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    mosquitofish; estrogen receptor; aryl hydrocarbon receptor; landfill wastewater;

    机译：蚊子鱼雌激素受体芳烃受体垃圾填埋场废水;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号