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首页> 外文期刊>Environmental toxicology and chemistry >MITOCHONDRIAL DNA COPY NUMBER AND hnRNP A2/B1 PROTEIN: BIOMARKERS FOR DIRECT EXPOSURE OF BENZENE
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MITOCHONDRIAL DNA COPY NUMBER AND hnRNP A2/B1 PROTEIN: BIOMARKERS FOR DIRECT EXPOSURE OF BENZENE

机译：线粒体DNA拷贝数和hnRNP A2 / B1蛋白：直接接触苯的生物标志物

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摘要

The present study was performed to identify biomarkers for exposure of benzene in blood cells and hematopoietic tissues. Peripheral mononuclear cells, hematopoietic stem cells, and leukemia cell lines were cultured in RPMI1640 media with the addition of 0, 1, and 10 mM of benzene. Hydrogen peroxide was measured using an enzyme immunoassay. Mitochondrial mass, membrane potential, and mitochondrial DNA (mtDNA) copy number were measured using MitoTracker Green/Red probes, and real-time polymerase chain reaction. In addition, two-dimensional gel electrophoresis and mass spectrometry matrix-assisted laser desorption/ ionization time-of-flight (MALDI-TOF) technology were performed to identify protein markers. The mitochondrial contents and membrane potentials were dramatically increased after three weeks of direct benzene exposure. The hydrogen peroxide level increased significantly after two weeks of treatment with benzene (4.4 ± 1.9μM/mg protein) compared to the non-benzene treatment group (1.2 ± 1.0; p = 0.001). The mtDNA copy number gradually increased after exposure to benzene. Numerous protein markers showed significant aberrant expression after exposure to benzene. Among them, the heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 was markedly decreased after exposure to benzene. Thus, increased mitochondrial mass, mtDNA copy number, and the hnRNP A2/B1 protein were biomarkers for benzene-related toxicity and hematotoxicity.
机译：进行本研究以鉴定血细胞和造血组织中苯暴露的生物标志物。在添加了0、1和10 mM苯的RPMI1640培养基中培养外周单核细胞，造血干细胞和白血病细胞系。使用酶免疫测定法测量过氧化氢。使用MitoTracker Green / Red探针和实时聚合酶链反应测量线粒体质量，膜电位和线粒体DNA（mtDNA）拷贝数。此外，还进行了二维凝胶电泳和质谱辅助激光解吸/电离飞行时间（MALDI-TOF）技术来鉴定蛋白质标记。直接接触苯三周后，线粒体含量和膜电位显着增加。与非苯处理组（1.2±1.0; p = 0.001）相比，用苯处理两周后（4.4±1.9μM/ mg蛋白），过氧化氢水平显着增加。接触苯后，mtDNA拷贝数逐渐增加。暴露于苯后，许多蛋白质标记物均显示出明显的异常表达。其中，异质核核糖核蛋白（hnRNP）A2 / B1暴露于苯后明显减少。因此，增加的线粒体质量，mtDNA拷贝数和hnRNP A2 / B1蛋白是苯相关毒性和血液毒性的生物标志物。

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  • 来源
    《Environmental toxicology and chemistry》 |2011年第12期|p.2762-2770|共9页
  • 作者

    Ha-Young Eom; Hye-Ran Kim; Hwan-Young Kim; Dong-Kyun Han; Hee-Jo Baek; Jae-Hyuk Lee; Jai Dong Moon; Jong-Hee Shin; Soon-Pal Suh; Dong-Wook Ryang; Hoon Kook; Myung-Geun Shin;

  • 作者单位

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Center for Biomedical Human Resources at Chonnam National University, Gwangju, Korea;

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Department of Pathology, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

    Department of Laboratory Medicine, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Hwasun, Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    hematotoxicity; benzene; biomarker; mitochondria; hnRNP A2/B1;

    机译：血液毒性苯;生物标志物线粒体hnRNP A2 / B1;

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