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首页> 外文期刊>Environmental toxicology and chemistry >17α-ETHINYLESTRADIOL INDUCES AN IMBALANCE BETWEEN APOPTOSIS AND CELL PROLIFERATION TO SEX STEROID DISRUPTION IN A TESTIS CULTURE OF GUDGEON, GOBIO GOBIO
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17α-ETHINYLESTRADIOL INDUCES AN IMBALANCE BETWEEN APOPTOSIS AND CELL PROLIFERATION TO SEX STEROID DISRUPTION IN A TESTIS CULTURE OF GUDGEON, GOBIO GOBIO

机译：17α-乙炔雌二醇引起古德冈古斯邦的睾丸培养细胞凋亡与细胞增殖性雌激素紊乱之间的不平衡

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The aim of this study was to investigate the effect of the most potent xenoestrogen currently found in the environment, ethinylestradiol (EE2), on some physiological events occurring during early spermatogenesis of gudgeon (Gobio gobio), a common European fish species. Physiological pathways studies were apoptosis, cell proliferation, and steroidogenesis on sex steroids (testosterone [T], 11-ketotestosterone [11-KT], and 17β-estradiol [E2]). Testis pieces were cultured in vitro during 21 d at 10~(-4), 10~(-3), 10~(-2), 10~(-1), 1 and 10 μg/L of EE2 as well as in positive (10~(-1) μg/L of E2) and ethanol control medium. Apoptosis and cell proliferation displayed opposite responses related to the EE2 concentration. When apoptosis inhibition was observed, cell proliferation was induced at 10~(-2) and 10~(-1) μg/L of EE2 as well as in the positive control. In contrast, a massive cell death was detected for high EE2 concentrations (1 and 10 μg/L). Steroidogenesis was also disrupted in a dose-related manner. 11-Ketotestosterone was depressed at 10~(-2) and 10~(-1) μg/L of EE2 whereas E2 was detectable in the medium only at 10~(-3), 10~(-2), and 10~(-1) μg/L of EE2. High concentrations of T were detected in the medium at 10~(-3), 10~(-2), and 10~(-1) μg/L of EE2 but depressed at 1 and 10 μg/L of EE2. In conclusion, intermediate EE2 concentrations (10~(-2) and 10~(-1) μg/L) used in this experimental design have obviously disrupted early spermatogenesis, leading to an imbalance between cell death and cell proliferation in a sex steroid environment toward E2. The results of the present study could be the basis conditions for oocyte development within the testis of a common teleost fish under xenoestrogen exposure.
机译：这项研究的目的是调查目前在环境中发现的最强异种雌激素乙炔雌二醇（EE2）对在欧洲常见鱼类common鱼（Gobio gobio）早期生精过程中发生的某些生理事件的影响。生理途径研究是性类固醇（睾丸激素[T]，11-酮睾丸激素[11-KT]和17β-雌二醇[E2]）的凋亡，细胞增殖和类固醇生成。睾丸块在10 d（-4），10 d（-3），10 d（-2），10 d（-1），1和10μg/ L EE2的体外培养21 d以及阳性（10〜（-1）μg/ L E2）和乙醇对照培养基。凋亡和细胞增殖显示与EE2浓度相关的相反反应。当观察到细胞凋亡抑制作用时，以10〜（-2）和10〜（-1）μg/ L的EE2以及阳性对照诱导细胞增殖。相反，对于高EE2浓度（1和10μg/ L），检测到大量细胞死亡。类固醇生成也以剂量相关的方式被破坏。 EE2的10〜（-2）和10〜（-1）μg/ L抑制了11-酮睾酮，而培养基中仅在10〜（-3），10〜（-2）和10〜下可检测到E2。 （-1）μg/ L的EE2。在EE2为10〜（-3），10〜（-2）和10〜（-1）μg/ L的培养基中检测到高浓度的T，但在EE2为1和10μg/ L时降低了T。总之，该实验设计中使用的中等EE2浓度（10〜（-2）和10〜（-1）μg/ L）明显破坏了早期精子发生，导致性激素环境中细胞死亡与细胞增殖之间的失衡。前往E2。本研究的结果可能是异雌激素暴露下普通硬骨鱼睾丸内卵母细胞发育的基础条件。

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