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首页> 外文期刊>Environmental toxicology and chemistry >EFFECT ASSESSMENT OF THE HERBICIDE PARAQUAT ON A GREEN ALGA USING DIFFERENTIAL GENE EXPRESSION AND BIOCHEMICAL BIOMARKERS
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EFFECT ASSESSMENT OF THE HERBICIDE PARAQUAT ON A GREEN ALGA USING DIFFERENTIAL GENE EXPRESSION AND BIOCHEMICAL BIOMARKERS

机译：利用差异基因表达和生物化学生物标志物评估除草剂百草枯对绿藻的影响

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摘要

Effects of the herbicide paraquat were assessed on the green freshwater alga Chlamydomonas reinhardtii using different endpoints of toxicity. Cell concentration and growth rate were monitored, whereas flow cytometry was applied to determine changes in chlorophyll content, viability and presence of reactive oxygen species. Furthermore, a transcriptomics approach using microarray hybridizations was applied to elucidate the mechanisms of toxicity. The results reveal that paraquat concentrations above 0.25 μM induce toxic effects in C. reinhardtii, reflected in a significantly reduced growth rate and cell concentration with a corresponding median effective concentration (EC50) value of 0.26 μM. With increasing paraquat concentrations, an increase in cell volume was registered with a particle counter as well as in the forward scattering signal of flow cytometric measurements, which is a measure of cell size. Flow cytometry, moreover, showed an increase in reactive oxygen species with increasing exposure concentration, corroborating the general knowledge that this herbicide exerts its toxicity through the generation of oxidative stress. At the same time, several genes involved in oxidative stress defense mechanisms, such as L-ascorbate peroxidase, glutaredoxin, and a possible glutathione-S-transferase were differentially expressed, demonstrating the value of microarrays for elucidating possible mechanisms of toxicity. The fact that several genes were differentially expressed at paraquat concentrations that caused no adverse effects on higher levels of biological organization indicates that a transcriptomics approach allows for the detection of early effects, even before they become manifest at higher levels.
机译：使用不同的毒性终点评估了除草剂百草枯对绿色淡水藻类莱茵衣藻的影响。监测细胞浓度和生长速率，而应用流式细胞术确定叶绿素含量，生存力和活性氧种类的变化。此外，使用微阵列杂交的转录组学方法被用来阐明毒性的机制。结果表明，百草枯浓度高于0.25μM会诱导莱茵衣藻的毒性作用，这反映为生长速率和细胞浓度显着降低，相应的有效浓度中位数（EC50）值为0.26μM。随着百草枯浓度的增加，细胞体积的增加与颗粒计数器以及流式细胞仪测量的前向散射信号（表明细胞大小的度量）有关。此外，流式细胞仪显示活性氧随着暴露浓度的增加而增加，这证实了该除草剂通过产生氧化应激而发挥其毒性的一般认识。同时，涉及氧化应激防御机制的几个基因，如L-抗坏血酸过氧化物酶，谷胱甘肽毒素和可能的谷胱甘肽S-转移酶被差异表达，证明了微阵列在阐明毒性的可能机制方面的价值。几个基因在百草枯浓度下差异表达而不会对更高水平的生物组织造成不利影响的事实表明，转录组学方法甚至可以在其以更高的水平表现出来之前就可以检测到早期效应。

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  • 来源
    《Environmental toxicology and chemistry》 |2010年第4期|p.893-901|共9页
  • 作者

    An Jamers; Wim De Coen;

  • 作者单位

    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171,2020 Antwerp, Belgium;

    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171,2020 Antwerp, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    paraquat; algae; transcriptomics; flow cytometry;

    机译：百草枯藻类转录组学流式细胞仪;

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