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首页> 外文期刊>Environmental toxicology and chemistry >A Fish Bioassay To Evaluate The Toxicity Associated With The Ingestion Of Benzo[a]pyrene-contaminated Benthic Prey
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A Fish Bioassay To Evaluate The Toxicity Associated With The Ingestion Of Benzo[a]pyrene-contaminated Benthic Prey

机译：鱼类生物测定法，用于评估与苯并[a] py污染的底栖猎物的食入相关的毒性

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A bioassay was developed to assess the toxic effects of ingested prey contaminated by polycyclic aromatic hydrocarbons (PAHs) using the teleost Fundulus heterociitus as a predator and the polychaete Nereis virens as a benthic vector. Ten groups of nine male adult Fundulus were exposed for 21 d to 10 different diets of Nereis contaminated with benzo[a]pyrene (BaP) by spiking dead Nereis with BaP (spiked Nereis [SN] diets, 0-26 μg of BaP per gram dry wt) or by exposing living Nereis to a diet, to sediments, or to both contaminated with BaP (exposed Nereis [EN] diets, 0-16 μg/g dry wt). Another group was exposed to commercial fish food, used as reference diet. Condition and prevalence of histopathological changes were not affected. Exposure to the SN diets containing at least 3.5 μg of BaP per gram dry weight caused an induction of ethoxyresorufin-O-deethylase activity in the intestine but not in the liver. In contrast, fish exposed to the highest doses (≥ 13.4 μg of BaP per gram dry wt) had increased cellular proliferation rate in the liver but not in the intestine. Quantifiable levels of free BaP tetrol-like metabolites were detected in the bile of fish exposed to diets containing more than 6.8 μg/g dry weight of BaP, and exhibited a dose-response relationship in fish exposed to SN diets. For a similar dose of BaP, EN and SN diets had similar effects. Thus, the BaP metabolic products that could have been produced in Nereis apparently did not contribute to the biomarkers responses. This bioassay can be applied to a variety of prey contaminated naturally or experimentally with PAHs. The present study supports the use of intestinal biomarkers, in addition to hepatic biomarkers, in environmental monitoring to assess the impact of dietary exposure to PAHs.
机译：开发了一种生物测定法，用于评估被硬骨鱼眼食肉动物作为食肉动物，以多毛Ne鱼作为底栖生物的载体，评估被多环芳香烃（PAHs）污染的食入猎物的毒性作用。十组九个成年男性成年Fund鼠，通过用BaP掺入死的Nereis（掺入Nereis [SN]日粮，每克0-26μgBaP），暴露于10种不同饮食中的苯并[a] py（BaP）污染的Nereis中，持续21天。干重）或通过将活的Nereis暴露于饮食，沉积物或两者均受BaP污染（Nereis [EN]饮食暴露，0-16μg/ g干wt）。另一组暴露于商业鱼类食品中，用作参考饮食。组织病理学改变的状况和患病率不受影响。暴露于每克干重至少3.5μgBaP的SN日粮中，会引起肠中乙氧基试卤灵-O-脱乙基酶活性的诱导，而不是肝脏中的诱导。相反，暴露于最高剂量（≥13.4μgBaP /克干重）的鱼在肝脏中的细胞增殖速率增加，但在肠中则没有。在暴露于含有干重超过6.8μg/ g BaP的日粮的鱼的胆汁中检测到了定量的游离BaP tetrol样代谢物，并且在暴露于SN日粮的鱼中表现出剂量-反应关系。对于类似剂量的BaP，EN和SN日粮具有相似的作用。因此，本来可以在Nereis中产生的BaP代谢产物显然对生物标志物的反应没有贡献。该生物测定法可应用于多种自然或实验中被PAH污染的猎物。本研究支持在环境监测中使用肠道生物标志物和肝生物标志物来评估饮食中PAHs暴露的影响。

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