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首页> 外文期刊>Environmental toxicology and chemistry >TOXICOLOGICAL SAFETY ASSESSMENT OF GENETICALLY MODIFIED BACILLUS THURINGIENSIS WITH ADDITIONAL N-ACYL HOMOSERINE LACTONASE GENE
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TOXICOLOGICAL SAFETY ASSESSMENT OF GENETICALLY MODIFIED BACILLUS THURINGIENSIS WITH ADDITIONAL N-ACYL HOMOSERINE LACTONASE GENE

机译：遗传修饰的苏云金芽孢杆菌与附加的N-酰基紫苏氨酸内酯酶基因的毒理学安全性评估

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摘要

The aim of the present study is to evaluate the toxicology safety to mammals of a genetically modified (GM) Bacillus thuringiensis with an additional N-acyl homoserine lactones gene (aiiA), which possesses insecticidal activity together with restraint of bacterial pathogenicity and is intended for use as a multifunctional biopesticide. Safety assessments included an acute oral toxicity test and 28-d animal feeding study in Wistar rats, primary eye and dermal irritation in Zealand White rabbits, and delayed contact hypersensitivity in guinea pigs. Tests were conducted using spray-dried powder preparation. This GM product showed toxicity neither in oral acute toxicity test nor in 28-d animal feeding test at a dose of 5,000 mg/kg body weight. During the animal feeding test, there were no significant differences in growth, food and water consumption, hematology, blood biochemical indices, organ weights, and histopathology finding between rats in controls and tested groups. Tested animals in primary eye and dermal irritation and delayed contact hypersensitivity test were also devoid of any toxicity compared to controls. All the above results demonstrated that the GM based multifunctional B. thuringiensis has low toxicity and low eye and dermal irritation and would not cause hypersensitivity to laboratory mammals and therefore could be regarded as safe for use as a pesticide.
机译：本研究的目的是评估具有额外的N-酰基高丝氨酸内酯基因（aiiA）的苏云金芽胞杆菌（Bacillus thuringiensis）对哺乳动物的毒理学安全性，该基因具有杀虫活性并能抑制细菌的致病性，旨在用于用作多功能生物农药。安全性评估包括对Wistar大鼠进行急性口服毒性试验和28天动物喂养研究，对西兰大白兔的主要眼睛和皮肤刺激以及对豚鼠的延迟接触性超敏反应。使用喷雾干燥的粉末制剂进行测试。该转基因产品在口服急性毒性试验和28天动物喂养试验中均未显示毒性，剂量为5,000 mg / kg体重。在动物饲养试验中，对照组和试验组大鼠的生长，食物和水消耗，血液学，血液生化指标，器官重量和组织病理学发现之间无显着差异。与对照组相比，接受原发眼和皮肤刺激以及延迟接触超敏反应测试的动物也没有任何毒性。所有以上结果表明，基于GM的多功能苏云金芽孢杆菌具有低毒性，低眼睛和皮肤刺激性，并且不会引起对实验室哺乳动物的超敏反应，因此可以被认为是安全使用的农药。

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