...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >INFLUENCES OF 4-NONYLPHENOL ON DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 1 GENE EXPRESSION AND VITELLOGENIN MRNA INDUCTION OF ADULT RARE MINNOW (GOBIOCYPRIS RARUS)
【24h】

INFLUENCES OF 4-NONYLPHENOL ON DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 1 GENE EXPRESSION AND VITELLOGENIN MRNA INDUCTION OF ADULT RARE MINNOW (GOBIOCYPRIS RARUS)

机译：4-壬基酚对双倍性和罕见的MAB-3转录因子1基因表达和卵白蛋白MRNA诱导的成年稀有野生稻（GOBIOCYPRIRS RARUS）的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mab-3 of Caenorhabdilis elegans and doublesex of Drosophila are two sex-determining genes that are found to have only a common DNA binding motif called a doublesex and mab-3 (DM) domain among phyla. The DM domain regulates some aspects of sexual development such as yolk protein synthesis. In the present study, by using DM degenerate primers' 13 sequences of doublesex and mab-3-related transcription factor (dmrt), genes were cloned from genomic DNA of rare minnow (Gobiocypris rarus) and expression of dmrt 1 was detected only in testis of adult males. In addition, partial sequences of vitellogenin (vtg) and β-actin genes as comparative expression of genes were cloned from the female liver by degenerate primers based on conserved regions. The vtg mRNA in male liver was induced significantly in concentrations higher than 2 μg/L treatments, although adult rare minnow were exposed to nominal concentrations of 2, 10, 50, 250, and 1,250 μg/L 4-nonylphenol (4-NP) for 21 d. The dmrtl mRNA level was significantly downregulated in a dose-dependent manner in all exposure groups of males. Moreover, other toxic endpoints, including growth, gonadosomatic index, hepatosomatic index, and histopathology, were determined. However, significant effects were observed only at higher exposure concentrations. Therefore, sex-specific genes such as dmrtl can be used as a more sensitive biomarker for 4-NP and for discovering molecular mechanisms underlying toxic response.
机译：秀丽隐杆线虫的mab-3和果蝇的双性是两个决定性别的基因，发现它们在门中只有一个常见的DNA结合基序，称为双性和mab-3（DM）结构域。 DM结构域调节性发育的某些方面，例如卵黄蛋白合成。在本研究中，通过使用DM简并引物的13个双性和mab-3相关转录因子（dmrt）序列，从稀有min鱼（Gobiocypris rarus）的基因组DNA中克隆基因，并且仅在睾丸中检测到dmrt 1的表达。成年男性。此外，通过基于保守区的简并引物从雌性肝脏中克隆出卵黄蛋白原（vtg）和β-肌动蛋白基因的部分序列，作为基因的比较表达。雄性肝脏中的vtg mRNA浓度明显高于2μg/ L处理，尽管成年稀有min鱼暴露于标称浓度为2、10、50、250和1,250μg/ L的4-壬基酚（4-NP） 21 d。在所有男性暴露组中，dmrt1 mRNA水平均以剂量依赖性方式显着下调。此外，还确定了其他毒性终点，包括生长，性腺体指数，肝体指数和组织病理学。但是，只有在较高的暴露浓度下才能观察到明显的影响。因此，性别特异性基因如dmrtl可用作4-NP的更敏感的生物标记，并用于发现潜在的毒性反应分子机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号