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首页> 外文期刊>Environmental toxicology and chemistry >Does Subcellular Distribution In Plants Dictate The Trophic Bioavailability Of Cadmium To Porcellio Dilatatus (crustacea, Isopoda)?
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Does Subcellular Distribution In Plants Dictate The Trophic Bioavailability Of Cadmium To Porcellio Dilatatus (crustacea, Isopoda)?

机译：植物中的亚细胞分布是否决定镉对Dicellatus（甲壳纲，异足纲）的营养生物利用度？

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摘要

The present study examined how subcellular partitioning of Cd in plants with different strategies to store and detoxify Cd may affect trophic transfer of Cd to the isopod Porcellio dilatatus. The plant species used were Lactuca saliva, a horticultural metal accumulator species; Thlaspi caerulescens, a herbaceous hyperaccumulator species; and the nonaccumulator, T. arvense. Taking into account that differences in subcellular distribution of Cd in plants might have an important role in the bioavailability of Cd to a consumer, a differential centrifugation technique was adopted to separate plant leaf tissues into four different fractions: cell debris, organelles, heat-denatured proteins, and heat-stable proteins (metallothionein-like proteins). Plants were grown in replicate hydroponic systems and were exposed for 7 d to 100 μM Cd spiked with ~(109)Cd. After a 14-d feeding trial, net assimilation of Cd in isopods following consumption of T. caerulescens and T. arvense leaves reached 16.0 ± 2.33 and 21.9 ± 1.94 μg/g animal, respectively. Cadmium assimilation efficiencies were significantly lower in isopods fed T. caerulescens (10.0 ± 0.92%) than in those fed T. arvense (15.0 ± 1.03%). In further experiments, Cd assimilation efficiencies were determined among isopods provided with purified subcellular fractions of the three plants. On the basis of our results, Cd bound to heat-stable proteins was the least bioavailable to isopods (14.4-19.6%), while Cd bound to heat-denatured proteins was the most trophically available to isopods (34.4-52.8%). Assimilation efficiencies were comparable in isopods fed purified subcellular fractions from different plants, further indicating the importance of subcellular Cd distribution in the assimilation. These results point to the ecological relevance of the subcellular Cd distribution in plants, which directly influence the trophic transfer of Cd to the animal consumer.
机译：本研究研究了在具有不同存储和解毒镉策略的植物中，镉在细胞中的亚细胞分配如何影响镉向等足线虫Porcellio dilatatus的营养转移。所使用的植物物种是Lactuca saliva，一种园艺金属蓄积物种。拟南芥（Thlaspi caerulescens），一种草类超蓄积物种；以及非蓄积的T. arvense。考虑到镉在植物中的亚细胞分布差异可能对镉对消费者的生物利用度具有重要作用，因此采用差异离心技术将植物叶片组织分为四个不同部分：细胞碎片，细胞器，热变性蛋白质和热稳定蛋白质（类金属硫蛋白）。使植物在复制水培系统中生长，并暴露于掺有〜（109）Cd的100μMCd中7 d。经过为期14天的喂养试验，食用铜青虫和and草叶片后，等足动物中的Cd净同化分别达到16.0±2.33和21.9±1.94μg/ g。饲喂拟青霉的等足类动物的镉同化效率（10.0±0.92％）显着低于饲喂拟南芥的等足类动物（15.0±1.03％）。在进一步的实验中，在提供了三株植物纯化亚细胞部分的等足动物中，测定了镉的同化效率。根据我们的结果，与热稳定蛋白结合的镉对异足动物的生物利用度最低（14.4-19.6％），而与热变性蛋白结合的镉对异足动物的营养利用性最高（34.4-52.8％）。在异足动物饲喂来自不同植物的纯化亚细胞级分中，同化效率相当，这进一步表明亚细胞Cd分布在同化中的重要性。这些结果指出了植物中亚细胞Cd分布的生态相关性，这直接影响了Cd向动物食用者的营养转移。

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