...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >Comparative Lipid Peroxidation and Apoptosis in Embryo-Larval Zebrafish Exposed to 3 Azole Fungicides, Tebuconazole, Propiconazole, andMyclobutanil, at Environmentally Relevant Concentrations
【24h】

Comparative Lipid Peroxidation and Apoptosis in Embryo-Larval Zebrafish Exposed to 3 Azole Fungicides, Tebuconazole, Propiconazole, andMyclobutanil, at Environmentally Relevant Concentrations

机译：在与环境相关的浓度下，暴露于3种偶氮类杀真菌剂，戊唑醇，丙环唑和霉菌丁的胚胎-幼虫斑马鱼的脂质过氧化和凋亡比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Azole fungicides have entered the aquatic environment through agricultural and residential runoff. In the present study, we compared the off-target toxicity of tebuconazole, propiconazole, and myclobutanil using embryo-larval zebrafish as a model. The aim of the present study was to investigate the relative toxicity of tebuconazole, propiconazole, and myclobutanil using multiple-level endpoints such as behavioral endpoints and enzymatic and molecular biomarkers associated with their mode of action. Zebrafish embryos were exposed to azoles at environmentally relevant and high concentrations, 0.3, 1.0, and 1000 mu g/L, starting at 5 h postfertilization (hpf) up to 48 hpf, as well as 5 d postfertilization (dpf). Relative mRNA expressions of cytochrome P450 family 51 lanosterol-14 alpha-demethylase, glutathione S-transferase, caspase 9, phosphoprotein p53, and BCL2-associated X protein were measured to assess toxicity attributable to fungicides at the mRNA level, whereas caspase 3/7 (apoptosis) and 3,4-methylene dioxy amphetamine (lipid peroxidation) levels were measured at the enzymatic level. Furthermore, mitochondrial dysfunction was measure through the Mito Stress test using the Seahorse XFe24 at 48 hpf. In addition, light to dark movement behavior was monitored at 5 dpf using Danio Vision (R) to understand adverse effects at the organismal level. There was no significant difference in the light to dark behavior with exposure to azoles compared to controls. The molecular biomarkers indicated that propiconazole and myclobutanil induced lipid peroxidation, oxidative stress, and potentially apoptosis at environmentally relevant concentrations (0.3 and 1 mu g/L). The results from the mitochondrial respiration assay indicated a slight decrease in spare respiratory capacity with an acute exposure (48 hpf) to all 3 azoles at 1000 mu g/L. Based on the present results, propiconazole and myclobutanil are acutely toxic compared to tebuconazole in aquatic organisms at environmentally relevant concentrations. Environ Toxicol Chem 2019;38:1455-1466. (c) 2019 SETAC
机译：唑类杀真菌剂已通过农业和住宅径流进入水生环境。在本研究中，我们使用胚胎幼虫斑马鱼作为模型比较了戊唑醇，丙环唑和霉菌丁的脱靶毒性。本研究的目的是使用行为终点和与其作用方式相关的酶和分子生物标记物等多水平终点，研究戊唑醇，丙环唑和霉菌丁的相对毒性。从受精后5 h（hpf）开始直至48 hpf，以及受精后5 d（dpf），将斑马鱼胚胎以与环境相关的高浓度0.3、1.0和1000μg / L的唑暴露于环境中。测量了细胞色素P450家族51羊毛甾醇14α-脱甲基酶，谷胱甘肽S-转移酶，胱天蛋白酶9，磷蛋白p53和BCL2相关X蛋白的相对mRNA表达，以评估在mRNA水平上可归因于杀真菌剂的毒性，而胱天蛋白酶3/7在酶促水平下测定（细胞凋亡）和3，4-亚甲基二氧基苯丙胺（脂质过氧化）水平。此外，线粒体功能障碍是通过在48 hpf下使用Seahorse XFe24通过Mito Stress测试进行测量的。此外，使用Danio Vision（R）在5 dpf的条件下对明暗运动行为进行了监测，以了解在生物水平上的不利影响。与对照相比，暴露于唑类的明暗行为没有显着差异。分子生物标记表明，丙环唑和霉菌丁在环境相关浓度（0.3和1μg / L）下诱导脂质过氧化，氧化应激和潜在的细胞凋亡。线粒体呼吸分析的结果表明，急性暴露于所有48 mg / L的3种唑类（48 hpf）时，备用呼吸能力略有下降。根据目前的结果，在环境相关浓度下，丙环唑和霉菌丁与戊唑醇相比在水生生物中具有剧毒。 Environ Toxicol Chem 2019; 38：1455-1466。 （c）2019年SETAC

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号