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首页> 外文期刊>Environmental toxicology and chemistry >DEVELOPMENT OF A DAPHNIA MAGNA DNA MICROARRAY FOR EVALUATING THE TOXICITY OF ENVIRONMENTAL CHEMICALS
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DEVELOPMENT OF A DAPHNIA MAGNA DNA MICROARRAY FOR EVALUATING THE TOXICITY OF ENVIRONMENTAL CHEMICALS

机译：用于评估环境化学物质毒性的DAPHNIA MAGNA DNA微阵列的开发

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摘要

Toxic chemical contaminants have a variety of detrimental effects on various species, and the impact of pollutants on ecosystems has become an urgent issue. However, the majority of studies regarding the effects of chemical contaminants have focused on vertebrates. Among aquatic organisms, Daphnia magna has been used extensively to evaluate organism- and population-level responses of invertebrates to pollutants in acute toxicity or reproductive toxicity tests. Although these types of tests can provide information concerning hazardous concentrations of chemicals, they provide no information about their mode of action. Recent advances in molecular genetic techniques have provided tools to better understand the responses of aquatic organisms to pollutants. In the present study, we adapted some of the techniques of molecular genetics to develop new tools, which form the basis for an ecotoxicogenomic assessment of D. magna. Based on a Daphnia expressed sequence tag database, we developed an oligonucleotide-based DNA microarray with high reproducibility. The DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to several different chemicals: Copper sulfate, hydrogen peroxide, pentachlorophenol, or β-naphthoflavone. Exposure to these chemicals resulted in characteristic patterns of gene expression that were chemical-specific, indicating that the Daphnia DNA microarray can be used for classification of toxic chemicals and for development of a mechanistic understanding of chemical toxicity on a common freshwater organism.
机译：有毒化学污染物对各种物种有多种有害影响，污染物对生态系统的影响已成为迫在眉睫的问题。但是，有关化学污染物影响的大多数研究都集中在脊椎动物上。在水生生物中，大型蚤（Daphnia magna）已广泛用于评估急性毒性或生殖毒性测试中无脊椎动物对污染物的生物和种群水平响应。尽管这些类型的测试可以提供有关危险化学品浓度的信息，但它们不提供有关其作用方式的信息。分子遗传技术的最新进展为更好地了解水生生物对污染物的反应提供了工具。在本研究中，我们采用了分子遗传学的一些技术来开发新的工具，这些技术为D. magna的生态毒理基因组学评估奠定了基础。基于水蚤表达序列标签数据库，我们开发了具有高重现性的基于寡核苷酸的DNA微阵列。 DNA微阵列用于评估暴露于几种不同化学物质（硫酸铜，过氧化氢，五氯苯酚或β-萘黄酮）的新生水蚤的基因表达谱。暴露于这些化学物质会导致特定基因表达特征的模式具有化学特异性，这表明水蚤DNA芯片可用于对有毒化学物质进行分类并用于发展对常见淡水生物化学毒性的机理理解。

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