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首页> 外文期刊>Environmental toxicology and chemistry >GUT BACTERIAL COMMUNITY STRUCTURE (PORCELLIO SCABER, ISOPODA, CRUSTACEA) AS A MEASURE OF COMMUNITY LEVEL RESPONSE TO LONG-TERM AND SHORT-TERM METAL POLLUTION
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GUT BACTERIAL COMMUNITY STRUCTURE (PORCELLIO SCABER, ISOPODA, CRUSTACEA) AS A MEASURE OF COMMUNITY LEVEL RESPONSE TO LONG-TERM AND SHORT-TERM METAL POLLUTION

机译：肠道细菌的群落结构（POSCELLIO SCABER，ISOPODA，CRUSTACEA），作为对长期和短期金属污染的群落水平反应的一种测量

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摘要

Prokaryotes are of high importance in the assessment of environmental pollution effects. Due to fast responsiveness of bacterial communities to environmental physicochemical factors, it is difficult to compare results of bacterial community investigations on the temporal and spatial scale. To reduce the effects of variable physicochemical environmental conditions on bacterial microbiota when investigating the specific impact of contaminants on bacterial communities, we investigated the bacterial community in the gut of terrestrial isopods (Porcellio scaber, Isopoda, Crustacea) from clean and metal-polluted environments. Animals were collected from a chronically mercury-polluted site, a chronically multiple metal-(Cd, Pb, Zn) polluted site, and two reference sites. In addition, animals from an unpolluted site were laboratory exposed to 5 μg Hg/g food in order to compare the effect of acute and chronic Hg exposure. The bacterial gut microbiota was investigated by temporal temperature gradient gel electrophoresis (TTGE) and clone library construction based on polymerase chain reaction amplified 16S rRNA genes. The major bacterial representatives of the emptied gut microbiota in the animals from the chronically polluted environments seemed not affected when analyzed by TTGE. The detailed bacterial community structure investigated by 16S rRNA clone library construction, however, showed that the community from the Hg-polluted site also was affected severely (242.4 operational taxonomic units [OTU] in the polluted and 650.6 OTU in the unpolluted environment). When animals were acutely exposed to mercury, changes of bacterial community structures already were seen on TTGE profiles and no additional analysis was needed. We suggest the use of P. scaber gut bacterial community structure as a measure of effects caused by both long- and short-term exposure to pollution.
机译：原核生物在评估环境污染影响方面非常重要。由于细菌群落对环境物理化学因素的快速响应，很难在时间和空间尺度上比较细菌群落调查的结果。为了在调查污染物对细菌群落的具体影响时减少可变的理化环境条件对细菌菌群的影响，我们调查了来自清洁和金属污染环境的陆生等足动物（Porcellio scaber，Isopoda，甲壳纲）肠道中的细菌群落。从长期汞污染的地点，长期被多种金属（Cd，Pb，Zn）污染的地点和两个参考地点收集动物。另外，实验室将来自未污染场所的动物暴露于5μgHg / g食物，以比较急性和慢性Hg暴露的影响。通过瞬时温度梯度凝胶电泳（TTGE）研究了细菌的肠道菌群，并基于聚合酶链反应扩增了16S rRNA基因构建了克隆文库。用TTGE分析时，来自慢性污染环境的动物中空肠微生物群的主要细菌代表似乎没有受到影响。然而，通过16S rRNA克隆文库构建研究的详细细菌群落结构显示，来自Hg污染位点的群落也受到严重影响（在污染的环境中为242.4个操作分类单位[OTU]，在未污染的环境中为650.6 OTU）。当动物急性接触汞时，TTGE曲线上已经看到了细菌群落结构的变化，因此无需进行其他分析。我们建议使用P. scaber肠道细菌群落结构来衡量长期和短期暴露于污染所造成的影响。

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