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首页> 外文期刊>Environmental toxicology and chemistry >GILL METAL BINDING AND STRESS GENE TRANSCRIPTION IN BROWN TROUT (SALMO TRUTTA) EXPOSED TO METAL ENVIRONMENTS: THE EFFECT OF PRE-EXPOSURE IN NATURAL POPULATIONS
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GILL METAL BINDING AND STRESS GENE TRANSCRIPTION IN BROWN TROUT (SALMO TRUTTA) EXPOSED TO METAL ENVIRONMENTS: THE EFFECT OF PRE-EXPOSURE IN NATURAL POPULATIONS

机译：暴露于金属环境的褐鳟（Salmo trutta）中的金属结合和应力基因转录：天然种群中预先暴露的影响

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Brown trout (Salmo trutta) from two native populations from the R0ros area in Central Norway, acclimated in mining-affected habitats to different levels of Cd/Zn and Cu, together with trout from a nearby unaffected river (reference) were transferred to a nearby lake with higher levels of Cu, Cd, and Zn than those in their respective native rivers. This experiment was conducted to gain information about the underlying resistance mechanisms developed in fish exposed to metal environments. The focus was on gill metal accumulation and transcription of the metal-responsive stress genes metallothionein-A (MT-A), Cu/Zn-superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and heat shock protein 70 (HSP-70). The only shared response shown between the three groups after transfer were Cu accumulation and MT-A induction. The Cu-acclimated trout produced mucus to reduce the uptake of Cu into the gills. The MT-A levels were highest in the Cd/Zn-acclimated trout both before and after transfer. Before transfer, antioxidant transcription (SOD and GPx) was higher in gills of Cu-acclimated compared to the Cd/Zn-acclimated trout, but increased transcription of antioxidant stress genes was observed after transfer in both metal-acclimated groups. The metal-acclimated trout groups also showed an increase in the transcription of HSP-70. Compared to the reference population not previously exposed to metals, stress gene transcription increased faster in the metal-acclimated populations. The exception was induction of CAT, which appeared to be depressed after transfer in Cd/Zn-acclimated trout. The data indicate that acclimation to chronic metal exposure involves different strategies to cope with different metals and that these strategies involve both physiological mechanisms (mucus production) as well as metal-related stress gene transcription.
机译：来自挪威中部R0ros地区的两个本地种群的褐鳟（Salmo trutta），在受采矿影响的生境中适应了不同水平的Cd / Zn和Cu，并将来自附近未受影响河流的鳟鱼（参考）转移到附近湖泊中的铜，镉和锌含量高于各自的原生河流。进行该实验是为了获得有关暴露于金属环境的鱼类中潜在的抗性机制的信息。重点是g金属的积累和金属响应应激基因金属硫蛋白-A（MT-A），铜/锌超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx），谷胱甘肽还原酶（GR）的转录）和热休克蛋白70（HSP-70）。转移后三组之间显示的唯一共有反应是Cu积累和MT-A诱导。适应铜的鳟鱼会产生粘液，以减少Cu中对铜的吸收。转移前后，Cd / Zn适应性鳟鱼的MT-A含量最高。转移之前，与Cd / Zn适应的鳟鱼相比，Cu适应的ill的抗氧化剂转录（SOD和GPx）更高，但是两个金属适应组在转移后都观察到抗氧化剂胁迫基因的转录增加。适应金属的鳟鱼组还显示出HSP-70转录的增加。与先前未暴露于金属的参考种群相比，在适应金属的种群中，胁迫基因转录的增长更快。唯一的例外是CAT的诱导，在Cd / Zn适应的鳟鱼中转移后，CAT似乎被抑制了。数据表明，适应慢性金属暴露涉及应对不同金属的不同策略，并且这些策略涉及生理机制（粘液产生）以及与金属相关的应激基因转录。

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