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首页> 外文期刊>Environmental toxicology and chemistry >GENETIC IMPOVERISHMENT IN LABORATORY CULTURES OF THE TEST ORGANISM CHIRONOMUS RIPARIUS
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GENETIC IMPOVERISHMENT IN LABORATORY CULTURES OF THE TEST ORGANISM CHIRONOMUS RIPARIUS

机译:试验有机赤尾鱼实验室培养的遗传贫困

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摘要

Genetic diversity among laboratory and field populations of Chironomus riparius was investigated using microsatellite DNA analysis. Individuals of midge cultures reared in 10 different laboratories showed a clear reduction in the number of alleles per locus and in the level of heterozygosity compared to two natural populations sampled in southern Germany. To reconstruct the rate of genetic impoverishment under laboratory conditions, genetic diversity was monitored in a C. riparius laboratory population for 23 generations. Additionally, 11 populations originating from different laboratories were crossed, and genetic diversity was compared among pure, crossed, and natural populations. The results demonstrate that C. riparius strains used in standard toxicity tests exhibit low levels of genetic variability. In addition, our experiments show that refreshment of stocks with individuals from other laboratories is not sufficient to restore genetic variation. Potential consequences of genetic impoverishment and improvements for toxicological bioassays are discussed.
机译:利用微卫星DNA分析技术,对河岸线虫(Chironomus riparius)的实验室和田间种群的遗传多样性进行了研究。与在德国南部采样的两个自然种群相比,在10个不同实验室中饲养的中等文化个体显示出每个基因座的等位基因数量和杂合度水平明显降低。为了重建实验室条件下的遗传贫困率,在河豚梭菌实验室种群中监测了23代的遗传多样性。此外,对来自不同实验室的11个种群进行了杂交,并比较了纯种群,杂交种群和自然种群的遗传多样性。结果表明,在标准毒性试验中使用的河豚曲霉菌株显示出低水平的遗传变异性。此外,我们的实验表明,与来自其他实验室的个人一起进行库存更新不足以恢复遗传变异。讨论了遗传贫困的潜在后果以及毒理生物测定的改进。

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