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首页> 外文期刊>Environmental toxicology and chemistry >HISTIDINE-RICH GLYCOPROTEIN FROM THE HEMOLYMPH OF THE MARINE MUSSEL MYTILUS EDULIS L. BINDS CLASS A, CLASS B, AND BORDERLINE METALS
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HISTIDINE-RICH GLYCOPROTEIN FROM THE HEMOLYMPH OF THE MARINE MUSSEL MYTILUS EDULIS L. BINDS CLASS A, CLASS B, AND BORDERLINE METALS

机译：来自海洋贻贝Mytilus edulis L.的溶血的富含组氨酸的糖蛋白结合了A类，B类和边界金属

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摘要

Few studies have directly addressed the question of how metals (both essential and nonessential) are transported in the circulatory system of bivalve mollusks. One potential metal-transport protein, histidine-rich glycoprotein (HRG), has previously been isolated and characterized from the blood plasma of the marine mussel Mytilus edulis L. The present study was undertaken to investigate the extent to which mussel HRG can bind a variety of essential and nonessential metals in vitro, using immobilized metal-ion affinity chromatography (IMAC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The equilibrium metal speciation model MINTEQA2 was used to compute the amount of metal that bound to the IMAC packing material during the charging and initial wash steps. Results demonstrated that HRG can bind all seven of the metals tested (Ca, Cd, Hg, Mg, Ni, Pd, and Zn) and that HRG is the only metal-binding protein in IMAC eluents. Because HRG-metal binding strengths (log K_a) likely correspond with histidine-metal binding strengths, and because HRG is the predominant mussel plasma protein, the majority of each of the seven metals probably would be present in mussel blood as protein-bound metal rather than as free metal ion. The finding that a single mussel plasma protein may be responsible for binding all these metals raises important questions about how these different metals are subsequently transferred from HRG to different tissues of the mussel, where they may exhibit tissue-specific patterns of utilization, sequestration, elimination, and toxicity.
机译：很少有研究直接解决金属（必需和非必需）如何在双壳类软体动物的循环系统中运输的问题。先前已经从海洋贻贝Mytilus edulis L的血浆中分离并鉴定了一种潜在的金属转运蛋白，富含组氨酸的糖蛋白（HRG）。本研究旨在研究贻贝HRG可以结合多种变体的程度固定化的金属离子亲和色谱（IMAC）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对体外必需和非必需金属进行分离平衡金属形态模型MINTEQA2用于计算在装料和初始洗涤步骤过程中与IMAC填充材料结合的金属量。结果表明HRG可以结合所有7种测试金属（Ca，Cd，Hg，Mg，Ni，Pd和Zn），并且HRG是IMAC洗脱液中唯一的金属结合蛋白。因为HRG与金属的结合强度（log K_a）可能与组氨酸与金属的结合强度相对应，并且由于HRG是贻贝血浆蛋白的主要成分，所以七种金属中的每种金属中的大多数可能会以蛋白结合金属的形式存在于贻贝血液中比作为自由金属离子单个贻贝血浆蛋白可能负责结合所有这些金属的发现提出了一个重要的问题，即这些不同的金属随后如何从HRG转移到贻贝的不同组织中，在那里它们可能表现出组织特异性的利用，螯合，消除模式和毒性。

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