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首页> 外文期刊>Environmental toxicology and chemistry >Analysis of Dioxins in Contaminated Soils With the CALUX and CAFLUX Bioassays, an Immunoassay, and Gas Chromatography/High-Resolution Mass Spectrometry
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Analysis of Dioxins in Contaminated Soils With the CALUX and CAFLUX Bioassays, an Immunoassay, and Gas Chromatography/High-Resolution Mass Spectrometry

机译：CALUX和CAFLUX生物测定，免疫测定和气相色谱/高分辨率质谱法分析污染土壤中的二恶英

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摘要

The chemically activated luciferase expression assay, the chemically activated fluorescence expression assay, and the enzyme-linked mmunosorbent assay (ELISA) are all bioanalytical methods that have been used for the detection and quantification of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs). However, no comparisons of the results obtained by these three methods have been published analyzing identical replicates of purified sample extracts. Therefore, we have evaluated the performance of each of these methods for analyzing PCDD/Fs in aliquots of extracts from aged-contaminated soil samples and compared the results with those obtained by gas chromatography/high-resolution mass spectrometry (GC/HRMS). The quantitative performance was assessed and the effects of sample purification and data interpretation on the quality of the bioassay results were investigated. Results from the bioanalytical techniques were, in principle, not significantly different from each other or from the GC/HRMS data (p = 0.05). Furthermore, properly used, all of the bioanalytical techniques examined were found to be sufficiently sensitive, selective, and accurate to be used in connection with soil remediation activities when aiming at the remediation goal recommended by the U.S. Environmental Protection Agency (i.e., < 1,000 pg toxic equivalency/g). However, a site-specific correction factor should be applied with the use of the ELISA to account for differences between the toxic equivalency factors and the ELISA cross-reactivities of the various PCDD/F congeners, which otherwise might significantly underestimate the PCDD/F content.
机译：化学活化的萤光素酶表达测定，化学活化的荧光表达测定和酶联免疫吸附测定（ELISA）都是用于检测和定量多氯二苯并对二恶英和多氯二苯并呋喃（PCDD / Fs）。但是，尚未发表通过这三种方法分析纯化样品提取物的相同重复样品得到的结果的比较。因此，我们评估了上述每种方法在分析陈旧污染土壤样品提取物中等分试样中PCDD / Fs的性能，并将结果与​​通过气相色谱/高分辨率质谱（GC / HRMS）获得的结果进行了比较。评估了定量性能，并研究了样品纯化和数据解释对生物测定结果质量的影响。原则上，生物分析技术的结果彼此之间或与GC / HRMS数据之间无显着差异（p = 0.05）。此外，当针对美国环境保护署建议的修复目标（即，<1,000 pg）时，经过适当使用的所有检查过的生物分析技术均被发现具有足够的敏感性，选择性和准确性，可用于土壤修复活动。毒性当量/ g）。但是，应使用特定位点校正因子与ELISA结合使用，以解决各种PCDD / F同系物的毒性当量因子与ELISA交叉反应性之间的差异，否则可能会大大低估PCDD / F的含量。

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  • 来源
    《Environmental toxicology and chemistry》 |2007年第6期|1122-1129|共8页
  • 作者

    Malin Nording; Michael S. Denison; David Baston; Ylva Persson; Erik Spinnel; Peter Haglund;

  • 作者单位

    Environmental Chemistry, Umea University, SE-901 87 Umea, Sweden Swedish Defense Research Agency, SE-901 82 Umea, Sweden;

    Department of Environmental Toxicology, University of California, Davis. California 95616, USA;

    Department of Environmental Toxicology, University of California, Davis. California 95616, USA;

    Environmental Chemistry, Umea University, SE-901 87 Umea, Sweden;

    Environmental Chemistry, Umea University, SE-901 87 Umea, Sweden;

    Environmental Chemistry, Umea University, SE-901 87 Umea, Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    CALUX; CAFLUX; Enzyme-linked immunosorbent assay; soil; dioxin;

    机译：CALUX;CAFLUX;酶联免疫吸附测定;泥;二恶英;

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