• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental toxicology and chemistry >LEAD TOXICITY IN ALFALFA PLANTS EXPOSED TO PHYTOHORMONES AND ETHYLENEDIAMINETETRAACETIC ACID MONITORED BY PEROXIDASE, CATALASE, AND AMYLASE ACTIVITIES
【24h】

LEAD TOXICITY IN ALFALFA PLANTS EXPOSED TO PHYTOHORMONES AND ETHYLENEDIAMINETETRAACETIC ACID MONITORED BY PEROXIDASE, CATALASE, AND AMYLASE ACTIVITIES

机译：过氧化物酶，过氧化氢酶和淀粉酶活性监测的苜蓿植物暴露于植物激素和乙二胺基乙酸中的铅毒性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This manuscript describes the toxicity of lead in alfalfa plants treated with ethylenediaminetetraacetic acid (EDTA) and the phytohormones indole-3-acetic-acid (IAA), gibberellic acid (GA), and kinetin (KN), on catalase (CAT), ascorbate peroxidase (APOX), and total amylase activity (TAA). In all cases Pb was used at 40 mg/L; EDTA at 0.2 mM (equimolar to Pb); and IAA, GA, and KN at 1, 10, and 100 μM, respectively. An experiment containing Pb at 40 mg/L, 0.2 mM EDTA, and IAA and KN at 100 μM each was performed to determine changes in TAA. A control (plain nutrient solution) also was used for comparison. In all cases the treatments were performed in triplicate. Standard procedures were followed to determine the activity of the respective enzymes. After 10 d of exposure to the treatments, the leaves were harvested, homogenized, and centrifuged, and the supernatants were analyzed for CAT, APOX, and TAA. All determinations were performed in triplicate. The results demonstrated that CAT was reduced significantly (p ＜ 0.05) by all treatments containing Pb, IAA, and GA at 10 and 100 μM. However, only the treatments Pb/EDTA/KN at 1, 10, and 100 μM reduced the APOX. The TAA in leaves of alfalfa plants was increased significantly (p ＜ 0.05) by all treatments. Overall, the results suggest that the CAT tests showed no lead toxicity to the alfalfa seedlings. However IAA at 10 and 100 μM revealed toxicity to the CAT enzyme. In addition, the APOX tests exhibited no toxicity to the peroxidase enzyme with the exception of Pb/EDTA/KN treatments. Finally, the TAA tests showed high Pb/EDTA/phytohormone toxicity to the amylase enzyme in alfalfa seedlings.
机译：该手稿描述了用乙二胺四乙酸（EDTA）和植物激素吲哚-3-乙酸（IAA），赤霉素（GA）和激动素（KN）处理的苜蓿植物中铅对过氧化氢酶（CAT），抗坏血酸的毒性过氧化物酶（APOX）和总淀粉酶活性（TAA）。在所有情况下，铅的使用量均为40 mg / L； EDTA为0.2 mM（等于Pb）; IAA，GA和KN分别为1、10和100μM。进行了包含40 mg / L铅，0.2 mM EDTA以及IAA和KN分别为100μM的实验以确定TAA的变化。对照（纯营养液）也用于比较。在所有情况下，治疗均重复三次。遵循标准程序来确定各个酶的活性。暴露于处理10天后，收获叶片，均质化并离心，然后分析上清液中的CAT，APOX和TAA。所有测定均重复三次。结果表明，所有浓度分别为10和100μM的Pb，IAA和GA的处理，CAT均显着降低（p ＜0.05）。但是，只有浓度为1、10和100μM的Pb / EDTA / KN处理可降低APOX。所有处理均使紫花苜蓿植物叶片中的TAA显着增加（p ＜0.05）。总体而言，结果表明CAT试验对苜蓿幼苗没有铅毒性。但是，IAA在10和100μM下显示出对CAT酶的毒性。此外，除Pb / EDTA / KN处理外，APOX测试对过氧化物酶没有毒性。最后，TAA试验表明，苜蓿幼苗中的Pb / EDTA /植物激素对淀粉酶有很高的毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号