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首页> 外文期刊>Environmental toxicology and chemistry >HEPATIC BIOMARKER RESPONSES TO ORGANIC CONTAMINANTS IN FERAL CHUB (LEUCISCUS CEPHALUS)-LABORATORY CHARACTERIZATION AND FIELD STUDY IN THE SAVA RIVER, CROATIA
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HEPATIC BIOMARKER RESPONSES TO ORGANIC CONTAMINANTS IN FERAL CHUB (LEUCISCUS CEPHALUS)-LABORATORY CHARACTERIZATION AND FIELD STUDY IN THE SAVA RIVER, CROATIA

机译：肝生物标记物对胎儿粪便中的有机污染物的反应-克罗地亚萨瓦河的实验室表征和田间研究

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As a widely spread cyprinid fish species, the European chub (Leuciscus cephalus) has been used extensively in bio monitoring programs. However, no laboratory dose-response and/or time course studies related to applied biomarkers have been reported on chub yet. In order to address this issue, specimens of juvenile chub caught in September 2005 in the Sava River, Croatia, were laboratory exposed to various (0.25-50 mg/kg) doses of either model polycyclic aromatic hydrocarbons (PAH) premutagen benzo[a]pyrene (BaP), or β-naphthoflavone (β-NF), a well-known model cytochrome 1A (CYP1A) inducer, for 3 (BaP) or 5 d (β-NF). The responses of several hepatic biomarkers were determined in the exposed fish: The hepatic 7-ethoxyresorufin-O-deefhylase activity, CYP1A content, glutathione 5-transferase (GST) activity, liver bioactivation potential, and the amount of hydroxylated polycyclic aromatic hydrocarbon bile metabolites determined by the fixed wavelength fluorescence and the high-performance liquid chromatography technique. The relevance of determined biomarker responses has been analyzed further and crosscorrelated with the same set of biomarkers, as well as with tissue concentrations of polycyclic aromatic hydrocarbons and polychlorinated biphenyls, determined in chub specimens collected in September 2005 at five different polluted locations along the Sava River. The species-specific upper and lower limits in responses of studied biomarkers were determined and the obtained ranges successfully evaluated in real field situation. With the exception of the GST activity, all other biomarkers determined in chub proved to be valuable indicators of environmental pollution. Finally, the results of the present study demonstrated that the same strategy of laboratory characterization in combination with field evaluation should be used regularly in the selection of optimal biomarkers and indicator species.
机译：作为一种广泛传播的塞浦路斯鱼类，欧洲European（Leuciscus cephalus）已在生物监测计划中得到广泛使用。但是，目前尚无关于与应用生物标志物有关的实验室剂量反应和/或时程研究的文献。为了解决这个问题，2005年9月在克罗地亚的萨瓦河捕获的少年石蜡标本在实验室中暴露于各种剂量（0.25-50 mg / kg）的两种模型多环芳烃（PAH）前诱变苯并[a] （（BaP）或β-萘黄酮（β-NF），一种众所周知的模型细胞色素1A（CYP1A）诱导剂，持续3（BaP）或5 d（β-NF）。在暴露的鱼中确定了几种肝生物标记物的反应：肝中的7-乙氧基间苯二酚-O-去氢酶活性，CYP1A含量，谷胱甘肽5-转移酶（GST）活性，肝脏生物激活潜力以及羟基化多环芳烃胆汁代谢产物的量由固定波长荧光和高效液相色谱技术测定。已确定的生物标志物反应的相关性已得到进一步分析，并且与同一组生物标志物以及2005年9月在萨瓦河沿岸五个不同污染地点采集的样品中测定的多环芳烃和多氯联苯的组织浓度相互关联。 。确定了所研究生物标志物响应的物种特异性上限和下限，并成功地评估了所获得的范围在实际情况下。除GST活性外，在chub中测定的所有其他生物标志物均被证明是环境污染的重要指标。最后，本研究的结果表明，在选择最佳生物标志物和指示剂物种时，应定期使用相同的实验室表征策略与现场评估相结合。

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