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首页> 外文期刊>Environmental toxicology and chemistry >METALLOTHIONEIN GENE EXPRESSION AND PROTEIN LEVELS IN TRIPLOID AND DIPLOID OYSTERS CRASSOSTREA GIGAS AFTER EXPOSURE TO CADMIUM AND ZINC
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METALLOTHIONEIN GENE EXPRESSION AND PROTEIN LEVELS IN TRIPLOID AND DIPLOID OYSTERS CRASSOSTREA GIGAS AFTER EXPOSURE TO CADMIUM AND ZINC

机译：镉和锌暴露后三叶牡蛎和牡蛎壳中的金属硫蛋白基因表达和蛋白质水平。

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摘要

Quantitative real-time polymerase chain reaction (PCR) was used to compare for the first time the differential expression of metallothionein (MT) isoform genes, together with biosynthesis of the total MT proteins, in the gills of triploid and diploid juvenile Pacific oyster Crassostrea gigas in response to cadmium (Cd) and zinc (Zn) exposure. Oysters were exposed to Cd (0.133 μM), Zn (15.3 μM), and Cd+Zn for 14 d. Results showed similar response capacities to metal exposures in the two populations. No significant difference was revealed in terms of MT gene expression, MT protein synthesis, and Cd accumulation. However, triploid oysters bioaccumulated Zn 30% less efficiently than diploid oysters. Among the three MT isoform genes, CgMT2 appeared to be more expressed than CgMT1, whereas CgMT3 appeared to be anecdotal (10~6 times lower than CgMT2). CgMT2 and CgMT1 gene expression levels were increased sevenfold in the presence of Cd, whereas Zn appeared to have no effect. A twofold increase in MT protein levels occurred in response to Cd exposure. Discrepancies between mRNA and protein levels suggest that in C. gigas MT are regulated at the transcriptional level, as well as at the translational level.
机译：实时定量聚合酶链反应（PCR）用于首次比较三倍体和二倍体幼体Pacific牡蛎Crassostrea gigas ill中金属硫蛋白（MT）同工型基因的差异表达以及总MT蛋白的生物合成。响应镉（Cd）和锌（Zn）暴露。将牡蛎暴露于Cd（0.133μM），Zn（15.3μM）和Cd + Zn 14天。结果表明，两个人群对金属暴露的响应能力相似。在MT基因表达，MT蛋白合成和Cd积累方面没有发现显着差异。但是，三倍体牡蛎比二倍体牡蛎生物富集锌的效率低30％。在三个MT同工型基因中，CgMT2似乎比CgMT1表达更多，而CgMT3似乎是轶事的（比CgMT2低10〜6倍）。在Cd存在下，CgMT2和CgMT1基因表达水平增加了七倍，而Zn似乎没有作用。响应Cd暴露，MT蛋白水平增加了两倍。 mRNA和蛋白质水平之间的差异表明，在C. gigas中，MT受转录水平和翻译水平的调节。

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