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首页> 外文期刊>Environmental toxicology and chemistry >ALTERATION OF NORMAL CELLULAR PROFILES IN THE SCLERACTINIAN CORAL (POCILLOPORA DAMICORNIS) FOLLOWING LABORATORY EXPOSURE TO FUEL OIL
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ALTERATION OF NORMAL CELLULAR PROFILES IN THE SCLERACTINIAN CORAL (POCILLOPORA DAMICORNIS) FOLLOWING LABORATORY EXPOSURE TO FUEL OIL

机译：实验室暴露于燃油后巩膜巩膜珊瑚（绒毛膜圆球）正常细胞形态的改变

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摘要

Petroleum contamination from oil spills is a continuing threat to our ocean's fragile ecosystems. Herein, we explored the effects of the water-soluble fraction of crude oil on a stony coral, Pocillopora damicornis (Linneaeus 1758). We developed methods for exposing corals to various concentrations of crude oil and for assessing the potential molecular responses of the corals. Corals were exposed to water-accommodated fraction solutions, and appropriate cellular biomarkers were quantified. When compared to the "healthy" control specimens, exposed corals exhibited shifts in biomarker concentrations that were indicative of a shift from homeostasis. Significant changes were seen in cytochrome P450 1-class, cytochrome P450 2-class, glutathione-5-transferase-pi,and cnidarian multixenobiotic resistance protein-1 biomarkers, which are involved the cellular response to, and manipulation and excretion of, toxic compounds, including polycyclic aromatic hydrocarbons. A shift in biomarkers necessary for porphyrin production (e.g., protoporphyrinogen oxidase IX and ferrochelatase) and porphyrin destruction (e.g., heme oxygenase-1 and invertebrate neuroglobin homologue) illustrates only one of the cellular protective mechanisms. The response to oxidative stress was evaluated through measurements of copper/zinc superoxide dismutase-1 and DNA glycosylase MutY homologue-1 concentrations. Likewise, changes in heat shock protein 70 and small heat shock proteins indicated an adjustment in the cellular production of proteins. Finally, the results of this laboratory study were nearly identical to what we observed previously among corals of a different species, Porites lobata, exposed to an oil spill in the field after the grounding of the Merchant Vessel Kyowa Violet.
机译：漏油造成的石油污染继续威胁着我们海洋脆弱的生态系统。在这里，我们探讨了原油的水溶性馏分对石珊瑚Pocillopora damicornis（Linneaeus 1758）的影响。我们开发了将珊瑚暴露于各种浓度的原油中以及评估珊瑚潜在分子响应的方法。将珊瑚暴露于水可容纳的馏分溶液中，并对适当的细胞生物标记物进行定量。当与“健康”对照标本进行比较时，裸露的珊瑚表现出生物标志物浓度变化，这表明从体内稳态发生变化。细胞色素P450 1类，细胞色素P450 2类，谷胱甘肽-5-转移酶-pi和刺胞多异生抗药性抗蛋白1生物标志物发生了显着变化，这些变化涉及细胞对有毒化合物的反应以及其操纵和排泄。 ，包括多环芳烃。卟啉产生所必需的生物标志物（例如原卟啉原氧化酶IX和亚铁螯合酶）和卟啉破坏（例如血红素加氧酶-1和无脊椎动物神经球蛋白同源物）的变化仅说明了细胞保护机制之一。通过测量铜/锌超氧化物歧化酶-1和DNA糖基化酶MutY同系物1浓度来评估对氧化应激的响应。同样，热激蛋白70和小的热激蛋白的变化表明蛋白质细胞产生的调节。最后，这项实验室研究的结果与我们先前在商船共和紫罗兰接地后暴露于田间漏油的不同物种珊瑚（Porites lobata）的珊瑚中观察到的结果几乎相同。

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