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Development and Application of a Microplate Assay for Toxicity Testing on Aquatic Cyanobacteria

机译：用于水生蓝藻毒性测试的微孔板检测方法的开发和应用

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Regulatory environmental risk assessment, applied to establish a protection limit for all bacterial diversity in surface waters, relies on a growth inhibition test performed on a single species of cyanobacteria and the activated sludge respiration inhibition test. Recently, the ability of this approach to protect adequately for bacteria that provide important ecosystem services has been questioned, and empirical data on additional species to further investigate the effectiveness of the environmental risk assessment are urgently required. We present the development and validation of a cost-effective and time-efficient microplate assay that is comparable to the traditional shake flask test for measurement of cyanobacteria growth rate after chemical exposure. The assay has been optimized to ensure that comparisons of cyanobacteria sensitivity under exponential growth are assessed across equivalent experimental conditions using phycocyanin fluorescence as a surrogate for cell density. The test system is validated using potassium dichromate, and the results are compared with those obtained in an Organisation for Economic Co-operation and Development (2011) test guideline 201 shake flask test system. This assay is suitable for the screening of new and legacy chemicals (including antibiotics) for which ecotoxicology data are lacking across a wide range of cyanobacteria, with the aim of developing more comprehensive environmental risk assessment. Environ Toxicol Chem 2020;00:1-16. (c) 2019 SETAC
机译：监管环境风险评估用于建立对地表水中所有细菌多样性的保护极限，它依赖于对单一种类的蓝细菌进行的生长抑制测试和活性污泥呼吸抑制测试。最近，这种方法对提供重要生态系统服务的细菌进行充分保护的能力受到质疑，迫切需要其他物种的经验数据，以进一步调查环境风险评估的有效性。我们介绍了一种经济高效且省时的微孔板检测方法的开发和验证，该方法可与传统摇瓶测试相媲美，用于测量化学暴露后的蓝藻生长速率。该测定已经过优化，以确保使用藻蓝蛋白荧光作为细胞密度的替代品，在相当的实验条件下评估指数增长下蓝细菌敏感性的比较。该测试系统使用重铬酸钾验证，并将结果与​​经济合作与发展组织（2011）测试指南201摇瓶测试系统获得的结果进行比较。该分析方法适用于筛查在广泛的蓝细菌范围内缺乏生态毒理学数据的新旧化学品（包括抗生素），目的是进行更全面的环境风险评估。环境毒性化学2020; 00：1-16。 （c）2019年SETAC

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