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首页> 外文期刊>Environmental toxicology and chemistry >QUANTITATIVE BIO-IMAGING ANALYSIS FOR EVALUATION OF SEXUAL DIFFERENTIATION IN GERM CELLS OF olvas-GFP/ST-Ⅱ YI MEDAKA (ORYZIAS LATIPES) NANOINJECTED IN OVO WITH ETHINYLESTRADIOL
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QUANTITATIVE BIO-IMAGING ANALYSIS FOR EVALUATION OF SEXUAL DIFFERENTIATION IN GERM CELLS OF olvas-GFP/ST-Ⅱ YI MEDAKA (ORYZIAS LATIPES) NANOINJECTED IN OVO WITH ETHINYLESTRADIOL

机译：乙炔雌二醇在卵母细胞中纳米注射的olvas-GFP /ST-Ⅱ异黄花鱼胚细胞中性分化的定量生物成像分析

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We examined the effects on sexual differentiation of nanoinjecting ethinylestradiol (EE_2) into embryos of olvas-GFP/ST-Ⅱ YI medaka (Oryzias latipes). This strain contains the green fluorescent protein (GFP) gene fused to the regulatory region of the medaka vasa gene, and germ-cell-specific expression of GFP can be visualized in living (transparent) individuals. The number of germ cells in untreated genotypic females (XX) was approximately 10-fold that in untreated genotypic males (XY) at 10 d posthatch (dph). Germ cell proliferation was prevented in XX females that developed from embryos nanoinjected with 0.5, 2.5, or 5.0 ng of EE_2. Some 10-dph XY males from embryos injected with 0.5 ng or more of EE_2 showed a larger fluorescent area and more germ cells than those of pooled control groups. Males and females from embryos injected with 5.0 ng of EE_2 had no significant difference in germ cell number or fluorescent area. Thus, EE_2 injection into embryos caused abnormal gonadal development in both sexes. Observations of external secondary sex characteristics and histological examination of adult gonads showed complete sex reversal in some males after 0.5-, 2.5-, and 5.0-ng treatments but no changes in XX females after any treatment. Thus, quantitative bio-imaging can aid in evaluating the effects of endocrine-disrupting chemicals on fish within 10 dph.
机译：我们研究了纳米注射乙炔雌二醇（EE_2）对olvas-GFP /ST-ⅡYI medaka（Oryzias latipes）胚胎的性别分化的影响。该菌株包含融合到medaka vasa基因调控区域的绿色荧光蛋白（GFP）基因，并且GFP的生殖细胞特异性表达可以在活的（透明）个体中观察到。孵化后10 d（dph），未经处理的基因型雌性（XX）生殖细胞的数量约为未经处理的基因型雄性（XY）的十倍。在XX个雌性中，生殖细胞的增殖被阻止，这些雌性是由用0.5、2.5或5.0 ng EE_2纳米注射的胚胎发育而来的。与混合对照组相比，注射了0.5 ng或更多EE_2的胚胎中约有10 dph XY雄性显示更大的荧光面积和更多的生殖细胞。注射5.0 ng EE_2的胚胎中的雄性和雌性在生殖细胞数量或荧光面积方面无显着差异。因此，将EE_2注入胚胎会导致两性的异常性腺发育。外部次要性别特征的观察和成年性腺的组织学检查显示，在0.5、2.5和5.0 ng治疗后，某些男性完全性逆转，但经过任何治疗后，XX雌性均无变化。因此，定量生物成像可以帮助评估破坏内分泌的化学物质对10 dph以内鱼类的影响。

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