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首页> 外文期刊>Environmental toxicology and chemistry >VITELLOGENIN DETECTION AND CHICK PATHOLOGY ARE USEFUL ENDPOINTS TO EVALUATE ENDOCRINE-DISRUPTING EFFECTS IN AVIAN ONE-GENERATION REPRODUCTION STUDY
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VITELLOGENIN DETECTION AND CHICK PATHOLOGY ARE USEFUL ENDPOINTS TO EVALUATE ENDOCRINE-DISRUPTING EFFECTS IN AVIAN ONE-GENERATION REPRODUCTION STUDY

机译：卵白蛋白的检测和小鸡病理学是评估人单代生殖研究中破坏内分泌作用的有用终点

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摘要

To investigate additional endpoints for screening of endocrine disrupters in birds, effects of 17β-estradiol (E2) on one-generation reproduction in the Japanese quail (Coturnix japonica) were assessed. Pairs of the 10-week-old Japanese quail were fed a low-phytoestrogen diet containing E2 at 0 (control), 10, 100, and 1,000 ppm for six weeks. In the E2 100- and 1,000-ppm groups, the parental quail represented marked toxic changes including high mortality, decreased food consumption, decreased gonad weights, gross and histologic toxic changes in the reproductive and other organs, and inhibition of the reproduction. However, no adverse effects were observed in the parental quail from the E2 10-ppm group. In the parental males, serum vitellogenin (VTG) concentrations were increased significantly in the E2 10-ppm group, disclosing that serum VTG concentration is one of the highly sensitive endpoints for evaluating estrogenic endocrine activities. In the E2 10-ppm group, number of eggs laid, number of eggs with abnormalities, eggshell strength and thickness, fertility, early and late viabilities of embryos, normal hatchling rate, and clinical signs, mortality, viability, and body weight of chicks at 14 d of age were not affected. However, histopathology of the chicks in the E2 10-ppm group revealed meaningful morphological changes in the reproductive organs, such as cystic dilatation of seminiferous tubules, increased interstitial cells in the testis, and decreased theca cells in the ovary. The present study suggests that serum VTG concentration in the parental quail and histopathology of reproductive organs in the offspring are sensitive endpoints and are useful as additional endpoints in the avian one-generation reproduction test using the Japanese quail for evaluating estrogenic endocrine-disrupting effects.
机译：为了研究筛选禽类内分泌干扰物的其他终点，评估了17β-雌二醇（E2）对日本鹌鹑（Coturnix japonica）一代繁殖的影响。对成对的10周龄的日本鹌鹑饲喂低E2含量（对照），10、100和1,000 ppm的E2，持续6周。在100 ppm和1,000 ppm E2组中，父母鹌鹑表现出明显的毒性变化，包括高死亡率，食物消耗减少，性腺重量降低，生殖器官和其他器官的总体和组织毒性变化以及对生殖的抑制作用。然而，在E2 10-ppm组的父母鹌鹑中未观察到不良反应。在亲本男性中，E2 10-ppm组的血清卵黄蛋白原（VTG）浓度显着增加，表明血清VTG浓度是评估雌激素内分泌活性的高度敏感的终点之一。在E2 10 ppm组中，产卵数，异常卵数，蛋壳强度和厚度，生育力，胚胎的早期和晚期生存力，正常孵化率以及临床体征，死亡率，生存力和雏鸡体重在14天时未受影响。然而，E2 10-ppm组的雏鸡的组织病理学显示生殖器官发生了有意义的形态变化，例如生精小管的囊性扩张，睾丸间质细胞增加以及卵巢中的theca细胞减少。本研究表明，父母鹌鹑中的血清VTG浓度和后代生殖器官的组织病理学是敏感的终点，并且在使用日本鹌鹑评估雌激素内分泌干扰作用的禽类一代生殖测试中，可用作其他终点。

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