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首页> 外文期刊>Environmental toxicology and chemistry >IDENTIFICATION OF SIX DIFFERENTIALLY EXPRESSED GENES IN RESPONSE TO COPPER EXPOSURE IN THE AQUATIC PLANT LEMNA GIBBA (DUCKWEED)
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IDENTIFICATION OF SIX DIFFERENTIALLY EXPRESSED GENES IN RESPONSE TO COPPER EXPOSURE IN THE AQUATIC PLANT LEMNA GIBBA (DUCKWEED)

机译：识别水生植物Limna Gibba（浮萍）中铜暴露的六种差异表达基因

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摘要

Aquatic plants are susceptible to metal pollution and provide an entry point for metals, such as copper, into the aquatic biosphere. Exposure of the aquatic plant Lemna gibba to copper has been associated with the production of reactive oxygen species (ROS) and oxidative damage, caused in large part by the ability of this metal to redox cycle. In particular, copper-mediated production of ROS, a known group of signaling molecules, triggers numerous defense responses in L. gibba. Therefore, the objective of the present study was to examine to what extent acute copper exposure alters gene expression. First, the kinetics of copper uptake was assessed to determine if assimilation occurred within the short exposures needed to induce gene expression. Subsequently, using differential display polymerase chain reaction, we identified six genes with expressions that were putatively altered in response to copper. Differential expression was confirmed by northern hybridization analysis and showed that copper causes an accumulation of transcripts that encode for callose synthase, heat shock protein 90, serine decarboxylase, and the biotin carboxylase subunit of acetyl—coenzyme A carboxylase. Conversely, copper caused a decline in transcript levels for genes encoding the HAP5 subunit of the heme-activated protein (HAP) transcription factor in addition to the chloroplast nucleoid DNA-binding protein CND41. Interestingly, the expressions of these genes are sensitive to cellular ROS levels. We believe that these gene products provide valuable information regarding the molecular mechanisms of copper toxicity.
机译：水生植物容易受到金属污染的影响，并为金属（例如铜）进入水生生物圈提供了切入点。水生植物Lemna gibba暴露于铜与活性氧（ROS）的产生和氧化损伤有关，这在很大程度上是由于该金属的氧化还原循环能力所致。具体地说，铜介导的ROS（一组已知的信号分子）的产生触发了吉氏乳杆菌的众多防御反应。因此，本研究的目的是研究急性铜暴露在多大程度上改变基因表达。首先，评估铜吸收的动力学以确定同化是否发生在诱导基因表达所需的短时间内。随后，使用差异显示聚合酶链反应，我们鉴定了六个基因，这些基因的表达被认为是响应铜而改变的。通过Northern杂交分析证实了差异表达，结果表明铜引起了转录物的积累，这些转录物编码call质合酶，热休克蛋白90，丝氨酸脱羧酶和乙酰辅酶A羧化酶的生物素羧化酶亚基。相反，除了叶绿体类核糖核酸结合蛋白CND41之外，铜还导致编码血红素激活蛋白（HAP）转录因子HAP5亚基的基因的转录水平下降。有趣的是，这些基因的表达对细胞ROS水平敏感。我们相信这些基因产物提供了有关铜毒性分子机制的有价值的信息。

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