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首页> 外文期刊>Environmental toxicology and chemistry >MICROBIAL CHARACTERIZATION OF ARTIFICIAL SEDIMENT AND COMPARISONS WITH NATURAL SEDIMENTS—IMPLICATIONS FOR TOXICITY TESTING
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MICROBIAL CHARACTERIZATION OF ARTIFICIAL SEDIMENT AND COMPARISONS WITH NATURAL SEDIMENTS—IMPLICATIONS FOR TOXICITY TESTING

机译：人工沉积物的微生物特征及其与天然沉积物的比较-毒性试验的意义

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The development and activity of microbiota in artificial sediment may have strong implications for the fate of test compounds and the outcome of toxicity tests. In this study, we compare a number of microbial variables in the artificial sediment commonly used in toxicity testing with that of natural sediments. Bacterial abundance of artificial sediment ranged 5.7 to 7.1 X 10~8 cells/g wet weight, which is about two orders of magnitude lower than values commonly reported for natural sediments. Similarly, alternative estimates of microbial biomass (sum of phospholipid fatty acid, ergosterol, adenosine triphosphate) were several times lower for artificial sediment compared with natural sediment. Bacterial activity (~3H-thymidine incorporation) ranged 4.0 to 7.4 pmol g~(-1) h~(-1) (or 0.062-0.113 μg C g~(-1) h~(-1)) in artificial sediments, which is low compared with values commonly reported for freshwater sediments. Community respiration in artificial sediment was 34 to 93 μg CO_2 g~(-1) d~(-1). Bacterial community composition assessed by terminal restriction fragment length polymorphism of polymerase chain reaction amplified 16S rRNA genes demonstrated that natural bacterial communities appear to be more diverse than their counterparts in artificial sediment. The average similarity of the microbial communities obtained by this method was less than 40%, and different operational taxonomic units appeared to dominate the artificial and natural sediment, respectively. These results and supporting data from previous studies in natural sediments suggest that the artificial sediment has a poorly developed microbial component that differs substantially from that in natural sediments.
机译：人工沉积物中微生物群的发育和活性可能对试验化合物的命运和毒性试验的结果产生重大影响。在这项研究中，我们比较了通常用于毒性测试的人工沉积物中的微生物变量与天然沉积物的微生物变量。人工沉积物的细菌丰度范围为5.7至7.1 X 10-8个细胞/克湿重，这比自然沉积物通常报道的值低约两个数量级。同样，人工沉积物的微生物生物量（磷脂脂肪酸，麦角固醇，三磷酸腺苷的总和）的替代估算值比天然沉积物低几倍。人工沉积物中的细菌活性（〜3H-胸苷掺入）为4.0至7.4 pmol g〜（-1）h〜（-1）（或0.062-0.113μgC g〜（-1）h〜（-1）），与通常报道的淡水沉积物的数值相比，该数值较低。人工沉积物中的群落呼吸为34〜93μgCO_2 g〜（-1）d〜（-1）。通过聚合酶链反应扩增的16S rRNA基因的末端限制性片段长度多态性评估的细菌群落组成表明，天然细菌群落比人工沉积物中的细菌群落更具多样性。通过这种方法获得的微生物群落的平均相似度小于40％，并且不同的操作分类单位似乎分别主导了人工和天然沉积物。这些结果和先前对天然沉积物研究的支持数据表明，人工沉积物具有较差的微生物成分，该微生物成分与天然沉积物存在很大差异。

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