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首页> 外文期刊>Environmental toxicology and chemistry >BIOTRANSFORMATION OF THE POLYCYCLIC AROMATIC HYDROCARBON PYRENE IN THE MARINE POLYCHAETE NEREIS VIRENS
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BIOTRANSFORMATION OF THE POLYCYCLIC AROMATIC HYDROCARBON PYRENE IN THE MARINE POLYCHAETE NEREIS VIRENS

机译：海洋多环线虫病毒中多环芳烃P的生物转化

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摘要

In vivo and in vitro biotransformation of the polycyclic aromatic hydrocarbon (PAH) pyrene was investigated in the marine polychaete Nereis virens. Assays were designed to characterize phase Ⅰ and Ⅱ enzymes isolated from gut tissue. High-pressure liquid chromatography measurement of 1-hydroxypyrene, pyrene-1-glucuronide, pyrene-1-sulfate, and pyrene-1-glucoside appeared to be a sensitive method for estimating the activity of pyrene hydroxylase, glucuronosyl transferase, and sulfotransferase. Total pyrene in gut tissue after a 5-d exposure to 10 μg/g dry weight pyrene constituted 65% pyrene-1-glucuronide, 12% pyrene-1-sulfate, 2% pyrene-1-glucoside, 4% 1-hydroxypyrene, and 17% pyrene, indicating that glucuronidation is the prominent phase Ⅱ pathway in this organism. Only pyrene hydroxylase activity was induced significantly after pre-exposure to sediment-associated pyrene (10 μg/g dry wt). Apparent kinetic parameters were determined for all enzymatic reactions. Glucuronidation was confirmed as being the prominent phase Ⅱ reaction, based on its high apparent maximum velocity (V_(max(a))). Sulfation had the lowest apparent Michaelis constant (K_(m(a))), indicating high specificity. Apparent kinetic parameters for pyrene hydroxylase activity were changed after induction with pyrene. Induced worms showed increased V_(max(a)) and decreased K_(m(a)) compared to noninduced worms, indicating that the relative amount of the cytochrome P450 enzyme(s) responsible for pyrene hydroxylation is increased. Our findings show that N. virens has a high biotransformation capacity for PAHs, with inducible pyrene hydroxylase activity, and that the most prominent phase Ⅱ pathway in this organism is glucuronidation.
机译：在海洋多毛小鸟Nereis virens中研究了多环芳烃（PAH）vivo的体内和体外生物转化。设计用于表征从肠组织分离的Ⅰ和Ⅱ期酶的分析方法。高压液相色谱法测量1-羟基py，-1--1-葡萄糖醛酸，pyr-1硫酸盐和pyr-1-葡萄糖苷似乎是估算sensitive羟化酶，葡糖醛酸糖基转移酶和磺基转移酶活性的灵敏方法。暴露于10μg/ g干重的weight 5天后，肠道组织中的总pyr构成65％-1--1-葡糖醛酸苷，12％2-1硫酸盐，2％-1--1-葡萄糖苷，4％1-羟基py， 17％的17，表明葡萄糖醛酸化是该生物中最主要的Ⅱ相途径。预暴露于沉积物相关的pyr（10μg/ g干重）后，仅significantly诱导羟化酶活性显着。确定所有酶促反应的表观动力学参数。葡萄糖醛酸化由于其最高表观最大速度（V_（max（a）））而被确认为主要的Ⅱ期反应。硫酸化具有最低的表观米氏常数（K_（m（a））），表明高特异性。用induction诱导后改变了hydroxyl羟化酶活性的表观动力学参数。与未诱导的蠕虫相比，诱导的蠕虫显示V_（max（a））增加，而K_（m（a））减少，表明负责pyr羟基化的细胞色素P450酶的相对量增加。我们的发现表明，N。virens对PAHs具有很高的生物转化能力，具有可诱导的pyr羟化酶活性，并且该生物中最主要的Ⅱ相途径是葡萄糖醛酸化。

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