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首页> 外文期刊>Environmental toxicology and chemistry >METALLOTHIONEIN-LIKE PROTEIN IN THE LEAST KILLIFISH HETERANDRIA FORMOSA AND ITS ROLE IN CADMIUM RESISTANCE
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METALLOTHIONEIN-LIKE PROTEIN IN THE LEAST KILLIFISH HETERANDRIA FORMOSA AND ITS ROLE IN CADMIUM RESISTANCE

机译：杀伤力最小的杂七叶甲中的类金属硫蛋白及其在抗镉中的作用

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摘要

Metallothioneins (MTs) are a class of low molecular weight, cysteine-rich, metal-binding proteins ubiquitous in animals. They function in metal regulation and detoxification. An MT-like protein was separated by gel-permeation high-performance liquid chromatography (HPLC) in the least killifish Heterandria formosa exposed to 6 mg/L of Cd for 26 h. Western blot analysis showed that this protein cross-reacted with a cod-MT antibody. We quantified MT-like protein levels by the Cd-hemoglobin saturation assay in fish from two Cd-resistant and two control laboratory populations. Fish from the resistant populations had higher MT-like protein levels than those from the control populations; this difference was found for basal levels and for levels after 26 h of exposure to Cd. Our results suggest that the MT-like protein detoxified Cd in the least killifish and accounted for at least part of the increased resistance in the Cd-resistant laboratory populations.
机译：金属硫蛋白（MTs）是一类在动物中普遍存在的低分子量，富含半胱氨酸的金属结合蛋白。它们在金属调节和排毒中起作用。 MT样蛋白通过凝胶渗透高效液相色谱（HPLC）在暴露于6 mg / L Cd 26小时的最小幼鱼福尔摩斯杂种中分离。蛋白质印迹分析表明该蛋白与cod-MT抗体交叉反应。我们通过来自两个抗Cd和两个控制实验室种群的鱼类中的Cd-血红蛋白饱和度测定对MT样蛋白水平进行了定量。来自抗性种群的鱼比对照种群的MT样蛋白质水平更高；在基础水平和暴露于镉的26小时后的水平中发现了这种差异。我们的结果表明，MT样蛋白可在最少的鱼类中使Cd排毒，并至少占耐Cd实验室种群中增加的抗性的一部分。

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