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首页> 外文期刊>Environmental toxicology and chemistry >HISTOPATHOLOGY AS A TOOL FOR THE EVALUATION OF ENDOCRINE DISRUPTION IN ZEBRAFISH (DANIO RERIO)
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HISTOPATHOLOGY AS A TOOL FOR THE EVALUATION OF ENDOCRINE DISRUPTION IN ZEBRAFISH (DANIO RERIO)

机译：组织病理学作为评估斑马鱼内分泌紊乱的工具（DANIO RERIO）

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The importance of histology as a tool in the evaluation of endocrine disruption in fish depends on the choice and interpretation of appropriate endpoints, as is illustrated by the analysis of the effects of exposure to the estrogen 17β-estradiol (E_2) and the nonaromatizable androgen 17-methyldihydrotestosterone (MDHT). The E_2 led to the disappearance of vitellogenic oocytes in the ovary and an increased area of relatively large, eosinophilic cells in the testis, which were identified as spermatogonia under high-power magnification; this was a relative increase, as was shown by histomorphometry, because of a decreased size of sper-matogenic cysts and a relative decrease of spermatocyte cysts. The E_2 also induced an accumulation of acidophilie fluid in vessels and interstitial spaces, confirmed by immunohistochemistry as vitellogenin, and basophilia in the liver also associated with the production of vitellogenin. The MDHT induced activation of Sertoli cells in the testis and a decreased presence of vitellogenic oocytes and a reduced growth of previtellogenic oocytes in the ovary. These observations indicate the advantages of examining multiple organ systems on whole-body sections and the application of adequate magnifications. Inclusion of additional techniques such as morphometry and immunohistochemistry is valuable to further uncover insidious effects of endocrine disruptors.
机译：组织学作为评估鱼类内分泌干扰的工具的重要性取决于对适当终点的选择和解释，如对雌激素17β-雌二醇（E_2）和不可芳香化的雄激素17暴露的影响分析所表明的那样-甲基二氢睾丸激素（MDHT）。 E_2导致卵巢中卵黄形成卵母细胞的消失，睾丸中较大的嗜酸性细胞的面积增加，在高倍镜下被鉴定为精原细胞。如组织形态计量学所显示的，这是相对增加，这是由于生精囊肿的大小减小和精细胞囊肿的相对减少。 E_2还诱导血管和间质间隙中嗜酸液体的积累，通过免疫组织化学证实为卵黄蛋白原，肝脏中的嗜碱性粒细胞也与卵黄蛋白原的产生有关。 MDHT诱导睾丸支持细胞的活化，卵黄原性卵母细胞的存在减少，卵巢中卵原性卵母细胞的生长减少。这些观察结果表明检查全身部位的多个器官系统以及应用适当放大倍数的优势。包括额外的技术，例如形态计量学和免疫组织化学，对于进一步揭示内分泌干扰物的隐匿作用是有价值的。

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