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首页> 外文期刊>Environmental toxicology and chemistry >USING MARINE BIOASSAYS TO CLASSIFY THE TOXICITY OF DUTCH HARBOR SEDIMENTS
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USING MARINE BIOASSAYS TO CLASSIFY THE TOXICITY OF DUTCH HARBOR SEDIMENTS

机译:使用海洋生物测定法对荷兰港口沉积物的毒性进行分类

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摘要

A procedure was developed to assess contaminated marine sediments from Dutch harbors for possible adverse biological effects using three laboratory bioassays: A 10-d survival test with the amphipod Corophium volutator, a 14-d survival test with the heart urchin Echinocardium cordatum (adults), and the bioluminescence inhibition test with the bacterium Vibrio fischeri (Microtox~(~R) solid phase test [SP]). Microtox results were mathematically corrected for the modifying influence of fine sediment particles. After a validation procedure on test performance and modifying factors, respectively, 81 %, 99%, and 90% of the amphipod, heart urchin, and Microtox results were approved. Lower and upper threshold limits for biological effects were set at respectively 24 and 30% mortality for C. volutator, 27 and 35% mortality for E. cordatum, and 24 and 48 toxic units for the Microtox SP based on significant differences with control sediment and the performance of reference sediments. The bioassays clearly distinguished harbor sediments that give rise to acute effects and those that do not. Threshold limits for the amphipods, heart urchins, and bacteria were exceeded in, respectively, 9 to 17%, 33 to 40%, and 23 to 50% of the sediment samples. Highest effects were observed in sediments from the northerly harbors; there was significantly less response in sediments from the Delta Region and the port of Rotterdam (The Netherlands). The procedure outlined in this paper can be used for routine screening of contaminated dredged material that is proposed for open water disposal.
机译:通过三种实验室生物测定法,开发了一种程序来评估荷兰港口受污染的海洋沉积物可能产生的不利生物影响:使用两栖动物Corophium v​​olutator进行的10天生存测试,使用心脏顽固的棘心棘心病(成人)进行的14天生存测试,以及用费氏弧菌进行的生物发光抑制测试(Microtox〜(〜R)固相测试[SP])。 Microtox结果经过数学校正,以改善细沙颗粒的影响。在对测试性能和修改因子进行验证之后,分别批准了两栖动物,心脏顽固病和Microtox结果的81%,99%和90%。根据与对照沉积物和参考沉积物的性能。生物测定法清楚地区分了引起急性影响的港口沉积物和没有引起急性影响的港口沉积物。在沉淀物中,两栖动物,心脏顽童和细菌的阈值分别超过了9%至17%,33%至40%和23%至50%。在北港的沉积物中观察到了最大的影响。来自三角洲地区和鹿特丹港(荷兰)的沉积物的响应明显减少。本文概述的程序可用于常规筛查建议用于露天水处理的受污染的疏material材料的常规筛查。

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